SAB4200720

Anti-β-Catenin antibody, Mouse monoclonal

clone 15B8, purified from hybridoma cell culture

• 別名: Anti-CTNNB1, Anti-armadillo
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: 15B8, monoclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~94 kDa
• 化学種の反応性: chicken, mouse, human, rat, monkey, hamster, bovine, canine
• 濃度: ~1.0 mg/mL
• テクニック: immunoblotting: 0.25-0.5 μg/mL using Madin-Darby bovine kidney (MDBK) cell line extract; immunofluorescence: 5-10 μg/mL using human breast adenocarcinoma MCF7 cells; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• アイソタイプ: IgG1
• UniProtアクセッション番号: P35222
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CTNNB1(1499) ; mouse ... Ctnnb1(12387) ; rat ... Ctnnb1(84353)

詳細

詳細

Anti-β-Catenin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the 15B8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with recombinant chicken β-Catenin. β-Catenin, also known as Catenin βfamily.

免疫原

recombinant chicken ß-Catenin

アプリケーション

Anti-β-Catenin antibody, Mouse monoclonal has been used in:

• immunoblotting
• immunofluorescence
• immunohistochemistry
• immunoprecipitation

生物化学的／生理学的作用

β-Catenin plays a dual function in the cell including cell-cell adhesion and as a component of the Wnt (wingless) signaling pathway. In adherens junctions, β-Catenin associates with the cytoplasmic domain of the cadherin family adhesion receptors, anchoring them to the actin cytoskeleton and thus acting as structural protein. β-Catenin interacts with T cell factor (TCF)- family transcription factors to activate target genes. Wnt/β-catenin signaling interacts with several cellular pathways in context-dependent ways, including JakSTAT (Janus kinase−Signal transducer and activator of transcription) cytokine, TGFβ (transforming growth factor−β), DNA damage and the Hippo signaling pathways. Furthermore, β-Catenin is involved in embryonic morphogenesis and tumorigenesis and is tightly regulated by phosphorylation and further degradation by the ubiquitin-proteasome system.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable