indirect immunofluorescence: 2.5-5 μg/mL using human HeLa cells. western blot: 2.5-5.0 μg/mL using whole extracts of HEK-293T cells over-expressing mouse NMD3.
NMD3 ribosome export adaptor (NMD3) is an adaptor for 60S subunit export via the export receptor CRM1 (XPO1). In human, the C-terminal domain of NMD3 comprises a CRM1- dependent leucine-rich nuclear export signal (NES) and a nuclear localization signal (NLS) in its. Two zinc-binding centres and the ribosome-binding domain forms the N-terminal domain.
免疫原
peptide corresponding to an internal region of human NMD3, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey, bovine and dog.
アプリケーション
Anti-NMD3 antibody produced in rabbit has been used in immunoblotting[1] and immunofluorescence.
生物化学的／生理学的作用
NMD3 ribosome export adaptor (NMD3) is a highly conserved nucleocytoplasmic shuttling protein, essential for nuclear export of 60S ribosomal subunits.
物理的形状
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
CRM1 is an export factor that together with its adaptor NMD3 transports numerous cargo molecules from the nucleus to cytoplasm through the nuclear pore. Previous studies have suggested that CRM1 and NMD3 are detected in the nucleolus. However, their localization
