SAB4200379

Anti-JMY antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• 別名: Anti-Junction mediating and regulatory protein, Anti-p53 cofactor
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~120 kDa
• 化学種の反応性: human
• 濃度: ~1.0 mg/mL
• テクニック: immunoprecipitation (IP): 5-10 μg using lysates of human WiDr cells.; indirect immunofluorescence: 1-2 μg/mL using human HeLa cells.; western blot: 0.5-1.0 μg/mL using whole extracts of human WiDr cells
• UniProtアクセッション番号: Q8N9B5
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... JMY(133746)

詳細

詳細

Junction mediating and regulatory protein (JMY) is identified as a transcriptional co-activator of p53 and is a multifunctional actin nucleation factor. JMY contains Wiskott-Aldrich syndrome protein (WASp)-homology 2 (WH2) domains in its C-terminus. The JMY gene is mapped to human chromosome location 5q14.1.

特異性

Anti-JMY recognizes human JMY.

免疫原

synthetic peptide corresponding to the C-terminal region of human JMY, conjugated to KLH.

アプリケーション

Anti-JMY antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence.

生物化学的／生理学的作用

Junction mediating and regulatory protein (JMY) acts as a nucleation promoting factor by activating actin-related proteins-2/3 (ARP2/3), creating branched filament networks, and also by assembling unbranched filaments directly through a Spire-like mechanism. Overexpression of JMY increases motility while its loss slows cell migration. DNA damage causes JMY to accumulate in the nucleus, where it enhances p53-dependent transcription of pro-apoptotic genes. The activities of JMY as a p53 co-factor or as an actin nucleation factor are regulated by its subcellular localization.

物理的形状

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

保管および安定性

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

免責事項

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable