SAB4200352

Anti-DLC1 (C-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody

• 別名: 抗ARHGAP7抗体, 抗FLJ21120抗体, 抗HP抗体, 抗STARD12抗体, 抗p122-RhoGAP抗体
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~200 kDa
• 交差性: human
• 強化検証: recombinant expression; Learn more about Antibody Enhanced Validation
• 濃度: ~1.5 mg/mL
• テクニック: western blot: 1-2 μg/mL using lysates of HEK-293T cells overexpressing human DLC1.
• UniProtアクセッション番号: Q96QB1
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... DLC1(10395)

詳細

詳細

Deleted in liver cancer 1 (DLC1) is a candidate tumor suppressor gene, originally obtained from human hepatocellular carcinoma (HCC). It is mapped to the human chromosome location 8p21-22. The DLC1 gene is majorly expressed in normal human tissues. It contains three major functional domains, sterile α-motif (SAM), Rho guanosine triphosphatase-activating protein (RhoGAP), and steroidogenic acute regulatory-related lipid transfer (START) domains. The N-terminal region of DLC1 localizes to focal adhesions by binding to Src Homology 2 (SH2) domains on tensins.

特異性

Anti-DLC1(C-terminal) specifically recognizes human DLC1.

免疫原

synthetic peptide corresponding to a sequence at the C-terminal of human DLC1, conjugated to KLH. The antibody is affinity-purified using the immunizing peptide immobilized on agarose.

アプリケーション

Anti-DLC1 (C-terminal) antibody produced in rabbit may be used in immunoblotting.

生物化学的／生理学的作用

Deleted in liver cancer 1 (DLC1) interacts with tensin2, a focal adhesion protein, localized at the end of stress fibers, which plays key roles in cytoskeletal organization and cell signaling. Mutations in the focal adhesion targeting (FAT) region of DLC1 reduces its expression and function. The focal adhesion localization of DLC1 is critical for cell motility and morphology. It is also critical for the tumor suppressor activity of DLC1 and under expressed in human hepatocellular carcinoma (HCC) and a variety of cancer types.

物理的形状

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

保管および安定性

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

免責事項

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable