immunoprecipitation (IP): 5-10 μg using lysates of human HeLa cells. western blot: 0.1-0.2 μg/mL using whole extracts of HEK-293 cells over-expressing human Golph3.
Golgi phosphoprotein 3 (Golph3) is a peripheral membrane protein of the Golgi stack that localizes to the trans-Golgi network. It is conserved from yeast to human. The GOLPH3 gene is mapped to the human chromosome 5p13.3 location.
特異性
Anti-Golph3 (C-terminal) recognizes human Golph3.
免疫原
peptide corresponding to the C-terminal region of human Golph3, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey and canine.
アプリケーション
Anti-Golph3 (C-terminal) antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.
生物化学的／生理学的作用
Golgi phosphoprotein 3 (Golph3) is a phosphatidylinositol-4-phosphate (PtdIns(4)P) binding protein required for Golgi function. It plays a vital role in Golgi trafficking and morphology by interacting with the unconventional myosin 18A (MYO18A) linking Golgi membranes to the F-actin cytoskeleton. GOLPH3 is an oncogene that is commonly targeted for amplification in human cancer and cancer cell lines. Furthermore, Golph3 overexpression is correlated with the hyperactivation of the mammalian target of rapamycin (mTOR) signaling in human cells.
物理的形状
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
保管および安定性
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
免責事項
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
