由来生物
rabbit
結合体
unconjugated
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
フォーム
buffered aqueous solution
分子量
antigen ~84 kDa
交差性
mouse, rat
濃度
~1.5 mg/mL
テクニック
immunoprecipitation (IP): 5-10 μg using rat brain extracts (S1 fraction)
indirect immunofluorescence: 5-10 μg/mL using NIH3T3 cells
western blot: 1.5-3 μg/mL using mouse brain extracts (S1 fraction)
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... GYS1(2997)
詳細
The glycogen synthase 1 (GYS1) gene is mapped to human chromosome 19q13.3. In mammals, two glycogen synthase (GS) isoforms have been identified, the muscle GS form, glycogen synthase 1 (GYS1) and the liver specific form, glycogen synthase 2 (GYS2, LGS). GYS1 is predominantly expressed in the skeletal and cardiac muscles.
特異性
Anti-Glycogen Synthase 1 (N-terminal), specifically recognizes mouse and rat glycogen synthase 1.
アプリケーション
Anti-Glycogen Synthase 1 (N-terminal) antibody produced in rabbit is suitable for:
- immunoblotting
- immunoprecipitation
- immunofluorescence
生物化学的／生理学的作用
Glycogen synthase (GS or GYS), the rate-limiting enzyme for glycogen biosynthesis, catalyzes the incorporation of α-1,4-linked glucose units into glycogen chains. Both, the muscle (GYS1) and the liver GS (GYS2) forms are highly regulated by phosphorylation, glucose availability, glycogen levels and allosteric effectors. In response to hormonal stimuli, GS is phosphorylated on up to nine serine residues resulting in progressive inactivation of the two isoforms. GS activity is stimulated by insulin in liver, muscle and adipose tissues. The enzyme undergoes dephosphorylation allosteric activation and thus translocates between various subcellular compartments. Insulin increases GS activity primarily by dephosphorylation of four key residues, a process thought to be mediated by the activation of protein phosphatase-1 (PP1) and the inactivation of glycogen synthase kinase-3 (GSK3). GYS1 deficiency might lead to congenital disorders associated with glycogen metabolism.
物理的形状
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
保管および安定性
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
適切な製品が見つかりませんか。
製品選択ツール.をお試しください
保管分類コード
10 - Combustible liquids
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
SAB4200218-200UL:
SAB4200218-25UL:
SAB4200218-BULK:
SAB4200218-VAR:
IXO14891:
最新バージョンのいずれかを選択してください：
Juan C Ferrer et al.
FEBS letters, 546(1), 127-132 (2003-06-28)
Traditionally, glycogen synthase (GS) has been considered to catalyze the key step of glycogen synthesis and to exercise most of the control over this metabolic pathway. However, recent advances have shown that other factors must be considered. Moreover, the control
T C Jensen et al.
The Journal of biological chemistry, 275(51), 40148-40154 (2000-10-03)
A protocol was developed in 3T3-L1 adipocytes that resulted in the specific desensitization of glycogen synthase activation by insulin. Cells were pretreated for 15 min with 100 nm insulin, and then recovered for 1.5 h in the absence of hormone.
María M Adeva-Andany et al.
BBA clinical, 5, 85-100 (2016-04-07)
In the human body, glycogen is a branched polymer of glucose stored mainly in the liver and the skeletal muscle that supplies glucose to the blood stream during fasting periods and to the muscle cells during muscle contraction. Glycogen has
Alexander von Wilamowitz-Moellendorff et al.
Diabetes, 62(12), 4070-4082 (2013-08-31)
The liver responds to an increase in blood glucose levels in the postprandial state by uptake of glucose and conversion to glycogen. Liver glycogen synthase (GYS2), a key enzyme in glycogen synthesis, is controlled by a complex interplay between the
アクティブなフィルタ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）
