S5447

抗SIRT1抗体 ウサギ宿主抗体

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• 別名: 抗S. cerevisiae, ホモログ様1抗体, 抗SIR2α抗体, 抗SIR2L1抗体, 抗SIR2抗体, 抗サーチュイン 1抗体
• MDL番号: MFCD08705602
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~120 kDa
• 化学種の反応性: human
• 濃度: ~1.0 mg/mL
• テクニック: immunoprecipitation (IP): 5-10 μg using extracts of Jurkat cells; western blot: 2.5-5 μg/mL using whole extracts of Jurkat cells
• UniProtアクセッション番号: Q96EB6
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... SIRT1(23411) ; mouse ... Sirt1(93759) ; rat ... Sirt1(309757)

詳細

詳細

Anti-SIRT1 recognizes human SIRT1. Sirtuin 1 (SIRT1) is encoded by the gene mapped to human chromosome 10q21.3. The encoded protein belongs to the silent information regulator (SIR) family and is a class of HDAC (histone deacetylase). SIRT1 is expressed in various tissues including brain, liver, pancreas, adipose tissue, and skeletal muscle. SIRT1 is also known as the longevity gene.

免疫原

ヒトSIRT1のアミノ酸580~593に相当する合成ペプチド (N末端システイン付加) のKLH結合体。相当する配列はラットおよびマウスSirt1では3アミノ酸が異なっています。

アプリケーション

Anti-SIRT1 antibody produced in rabbit has been used in immunohistochemistry.

生物化学的／生理学的作用

Sirtuin 1 (SIRT1) functions as a regulator of various metabolic pathways, and influences the pathophysiology of several metabolic diseases. It is a regulator of protein deacetylation, and is a candidate therapeutic target in non-alcoholic fatty liver disease (NAFLD), amyotrophic lateral sclerosis (ALS), kidney disease, and pulmonary disease. It also participates in tumorigenesis, and whether it functions as an oncogene or as a tumor suppressor depends upon the tumor type. In pancreatic ductal adenocarcinoma (PDAC), its elevated expression is linked with poor prognosis, and in non-small-cell lung cancer (NSCLC), it suppresses the expression of tumor suppressor p27. The encoded protein inhibits the terminal differentiation in dividing myocytes by binding to the myoD cofactor, pCAF. It is also thought to function as a suppressor of cardiovascular disorders, such as myocardial infarction, or neurodegenerative diseases, such as Alzheimer′s disease (AD) or Parkinson′s disorder (PD).

物理的形状

0.01 M PBS溶液 (pH 7.4, 15 mM アジ化ナトリウム含有)。

保管および安定性

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frostfree” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

その他情報

In order to obtain the best results using various techniques and preparations, we recommend determining the optimal working dilutions by titration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)