S8447

抗Sirt2 ウサギ宿主抗体

affinity isolated antibody, buffered aqueous solution

• 別名: 抗SIR2L2抗体, 抗SIR2L抗体, 抗サーチュイン (silent mating type information regulation2ホモログ) 抗体, 抗サーチュイン 2抗体
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD07784768
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IF, IP, WB
• Citations: 40

特性

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 37:43 kDa (rat), antigen 43 kDa (human)
• species reactivity: rat, mouse, human
• packaging: antibody small pack of 25 μL
• technique(s): immunoprecipitation (IP): 2-5 μg using extract of COS7 cells or 293 cells expressing recombinant human SIRT2 protein., indirect immunofluorescence: 5-10 μg/mL using human HeLa cells., western blot: 1-2 μg/mL using whole extracts of mouse and rat brain.
• UniProt accession no.: Q8IXJ6
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SIRT2(22933) ; mouse ... Sirt2(64383) ; rat ... Sirt2(361532)

詳細

General description

Rabbit polyclonal anti-Sirt2 antibody recognizes mouse, rat (37/43 kDa), and human (43 kDa) Sirt2. Detection of the Sirt2 bands by immunoblotting is specifically inhibited with the immunizing peptide.

Sirt2 is an NAD+-dependent tubulin deacetylase. Two Sirt2 isoforms exist as the result of alternative splicing. Sirt2 is overexpressed during mitosis and is multiply phosphorylated at the G2/M transition of the cell cycle, indicating that it plays a role in the control of mitotic exit in the cell cycle. Although Sirt2 resides predominantly in the cytoplasm, it becomes enriched in the nucleus during the cell cycle where it is associated with mitotic structures. Sirt2 was found to be an oligodendroglial protein that affects cell differentiation through deacetylating α-tubulin.

Sirtuin 2 (SIRT2) is a cytoplasmic protein, which is part of the sirtuin family of proteins. SIRT2 colocalizes with microtubules. This gene is located on human chromosome 19q13.2.

Immunogen

マウスSirt2のアミノ酸341~352に相当する合成ペプチドのKLH結合体。相当する配列はラットと同一であり、ヒトSirt2では1アミノ酸が異なっています。

Application

Anti-Sirt2 antibody has been used in western blotting and immunofluorescence.

Rabbit polyclonal anti-Sirt2 antibody may be used in several applications including immunoblotting, immunoprecipitation, and immunofluorescence.

Biochem/physiol Actions

Sirtuin 2 (SIRT2) maintains the integrity of the genome. Inhibition of SIRT2 can lead to neuroprotection in cellular and invertebrate models of Huntington′s disease. SIRT2 can deacetylate lys40 of α-tubulin both in vitro and in vivo. Knockdown of SIRT2 by small interfering RNA (siRNA) leads to tubulin hyperacetylation.

Physical form

0.01 M PBS溶液 (pH 7.4, 15 mM アジ化ナトリウム含有)。

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類: 10 - Combustible liquids
• PPE（個人用保護具）: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)