由来生物
mouse (PYS-2 cells)
アッセイ
~80% (SDS-PAGE)
フォーム
lyophilized powder
テクニック
cell culture | mammalian: suitable
表面被覆率
4‑40 μg/cm2
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
遺伝子情報
mouse ... Sparc(20692)
アプリケーション
様々な組織で発現され、in vitroで細胞の伸展を阻害し接着点の数を減少させます。
生物化学的／生理学的作用
アルブミン, コラ-ゲン, およびトロンボスポンジンと結合します。また、内皮細胞, 平滑筋細胞, および線維芽細胞の増殖を阻害します。
構成
SPARC, or Secreted Protein Acidic and Rich in Cysteine, is a 283-287 AA Ca2+ binding to glycoprotein with an apparent molecular weight of 40-43 kDa.
注意
The lyophilized powder should be stored frozen at -20°C. The reconstituted product should be stored frozen at -70°C.
調製ノート
This product is supplied as a lyophilized powder from phosphate buffered saline at 25 μg protein/vial. It should be reconstituted aseptically with sterile water, producing a solution of 0.1 to 0.3 mg protein per mL. SPARC is initially purified from mouse parietal yolk sac cells under conditions that preserve biological activity.
保管分類コード
13 - Non Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
S5174-VAR:
S5174-BULK:
S5174-25UG-PW:
S5174-25UG:
H Sage et al.
The Journal of cell biology, 109(1), 341-356 (1989-07-01)
SPARC (Secreted Protein Acidic and Rich in Cysteine) is a Ca+2-binding glycoprotein that is differentially associated with morphogenesis, remodeling, cellular migration, and proliferation. We show here that exogenous SPARC, added to cells in culture, was associated with profound changes in
G Chen et al.
British journal of cancer, 102(1), 188-195 (2009-11-19)
Secreted protein acidic and rich in cysteine (SPARC) is a multi-faceted protein-modulating cell-cell and cell-matrix interactions. In cancer, SPARC can be not only associated with a highly aggressive phenotype, but also acts as a tumour suppressor. The aim of this
T F Lane et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 8(2), 163-173 (1994-02-01)
An extracellular matrix-associated glycoprotein expressed in a variety of tissues during embryogenesis and repair, SPARC contains modular domains that can function independently to bind cells and matrix components. Because SPARC can selectively disrupt cellular contacts with matrix and thereby effect
Clément Faye et al.
The Journal of biological chemistry, 284(33), 22041-22047 (2009-06-23)
Endostatin is a C-terminal proteolytic fragment of collagen XVIII that is localized in vascular basement membrane zones in various organs. It binds to heparin/heparan sulfate and to a number of proteins, but its molecular mechanisms of action are not fully
資料
Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.
アクティブなフィルタ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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