形状
buffered aqueous glycerol solution
濃度
10,000 units/mL
輸送温度
wet ice
保管温度
−20°C
特異性
認識配列: 5'-C/CGG-3'
ライゲーション・再切断アッセイ:2～10倍のMsp Iで1 μgのλDNA基質を過剰消化した場合、切断率は100%、ライゲーション率は>95%、再切断率は>95%です。
熱失活:65°C、15分間で失活します。
ライゲーション・再切断アッセイ:2～10倍のMsp Iで1 μgのλDNA基質を過剰消化した場合、切断率は100%、ライゲーション率は>95%、再切断率は>95%です。
熱失活:65°C、15分間で失活します。
その他情報
Supplied with 10x Restriction Endonuclease Buffer SL (B3782).
物理的形状
10 mM Tris-HCl溶液(pH 7.5、1 mM EDTA、50 mM KCl、10 mM 2-メルカプトエタノール、50%グリセロール(v/v)、0.2%Triton X-100(v/v)含有、4°C)
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
C Waalwijk et al.
Nucleic acids research, 5(9), 3231-3236 (1978-09-01)
The cleavage of DNA by restriction endonucleases HpaII and HapII is prevented by the presence of a 5-methyl group at the internal C residue of its recognition sequence CCGG. MspI, an isoschizomer of HpaII available from New England Biolabs, cleaves
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
D S Jansson et al.
British poultry science, 53(6), 790-799 (2013-02-13)
1. Vancomycin-resistant Enterococcus faecium (VRE(fm)) has recently spread among Swedish broiler farms. The objectives were to investigate VRE(fm) persistence within barns between flocks, and to determine whether day-old chicks, feed or forklift trucks used for loading crates could be identified
Yu-Feng Huang et al.
BMC systems biology, 6 Suppl 2, S10-S10 (2013-01-11)
Current next-generation sequencing (NGS) platforms adopt two types of sequencing mechanisms: by synthesis or by ligation. The former is employed by 454 and Solexa systems, while the latter by SOLiD system. Although the pros and cons for each sequencing mechanism
Nico Mitro et al.
Methods in molecular biology (Clifton, N.J.), 952, 137-144 (2012-10-27)
The role of certain amino acids in the interactions of ligands with their cognate nuclear receptors is usually achieved by the resolution of the crystal structure of the receptor complexed with the ligand. As a complementary functional approach, site-directed mutagenesis
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