P4056

血小板由来成長因子-BB ラット由来

PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• 別名: PDGF-BB
• MDL番号: MFCD01094933
• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• リコンビナント: expressed in E. coli
• 品質水準: 100
• 形状: lyophilized powder
• 有効性: 1.62 ng/mL EC₅₀
• 品質: endotoxin tested
• 分子量: ~30 kDa
• 包装: pkg of 5x50 μg; pkg of 50 μg
• 保管条件: avoid repeated freeze/thaw cycles
• テクニック: cell culture | mammalian: suitable
• 不純物: ≤1 EU/μg
• UniProtアクセッション番号: P31240
• 保管温度: −20°C
• 遺伝子情報: rat ... Pdgfb(24628)

詳細

詳細

Platelet-derived growth factor (PDGF) is a 30 kDa cationic polypeptide and consists of two disulphide linked chains A and B. PDGF is expressed as hetero and homodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD.

アプリケーション

Platelet-Derived Growth Factor-BB from rat has been used as a growth supplement in mouse embryonic fibroblast culture, motoneuron-muscle co-culture and adult spinal cord neurons culture.

生物化学的／生理学的作用

Platelet-derived growth factor (PDGF) elicits multifunctional actions with a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells, and chondrocytes. PDGF is a potent chemoattractant and activator of neutrophils, monocytes, and fibroblasts. PDGF activates smooth muscle relocalization and multiplication. PDGF mediated increased smooth muscle muscle proliferation might be associated with gene regulation. It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins, and modulates LDL (low density lipoprotein) receptor binding.

その他情報

ラットPDGFのB鎖からなる二量体

物理的形状

0.2 μmフィルタ-で濾過した30%アセトニトリル、0.1%トリフルオロ酢酸、2.5 mg BSA溶液の凍結乾燥品

アナリシスノート

生物活性は、休止期のNR6R-3T-3線維芽細胞の³H-thymidine取り込みに対する促進活性として測定されています。

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)