MSST0047

SILu^™Prot SOST, Sclerostin human

recombinant, expressed in HEK 293 cells, SIL MS Protein Standard, ¹³C and ¹⁵N-labeled

• UNSPSCコード: 23201100
• NACRES: NA.12

特性

• 由来生物: human
• 品質水準: 200
• リコンビナント: expressed in HEK 293 cells
• タグ: 8-His tagged
• アッセイ: ≥98% (SDS-PAGE)
• フォーム: lyophilized powder
• 有効性: ≥98% Heavy amino acids incorporation efficiency by MS
• テクニック: mass spectrometry (MS): suitable
• 適合性: suitable for mass spectrometry (standard)
• UniProtアクセッション番号: Q9BQB4
• 保管温度: −20°C
• 遺伝子情報: human ... SOST(50964)

詳細

詳細

SILu^™Prot SOST is a recombinant, stable isotope-labeled human SOST which incorporates [¹³C₆, ¹⁵N₄]-Arginine and [¹³C₆, ¹⁵N₂]-Lysine. Expressed in human 293 cells, it is designed to be used as an internal standard for bioanalysis of SOST in mass-spectrometry. SILu^™Prot SOST is a protein of 201 amino acids (including a N-terminal polyhistidine and tag), with a calculated molecular mass of 22.8 kDa.

生物化学的／生理学的作用

Sclerostin is a secreted Wnt signaling antagonist produced almost exclusively by osteocytes. It can selectively inhibit Wnt/ β-catenin, suppressing the activity of osteoblasts as well as the viability of osteoblasts and osteocytes. Lower sclerostin levels are associated with lower bone mineral content and bone. It was demonstrated that greater total limb bone mineral content was significantly associated with greater circulating levels of sclerostin. In addition, circulating sclerostin is a biomarker of osteoporosis severity in long-term, chronic paraplegia. Serum sclerostin was associated significantly, independently, and positively with bone mineral density of both cortical and cancellous bone. Sclerostin is considered to be one of the factors associated with chronic kidney disease-mineral and bone disorder in hemodialysis patients.

シーケンス

HHHHHHHHGGQQGWQAFKNDATEIIPELGEYPEPPPELENNKTMNRAENGGRPPHHPFETKDVSEYSCRELHFTRYVTDGPCRSAKPVTELVCSGQCGPARLLPNAIGRGKWWRPSGPDFRCIPDRYRAQRVQLLCPGGEAPRARKVRLVASCKCKRLTRFHNQSELKDFGTEAARPQKGRKPRPRARSAKANQAELENAY

物理的形状

Supplied as a lyophilized powder containing phosphate buffered saline.

法的情報

SILu is a trademark of Sigma-Aldrich Co. LLC

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable