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詳細
Two leaders in genome editing, Sigma-Aldrich and the Wellcome Trust Sanger Institute, have joined forces to make the first ever arrayed lentiviral CRISPR knockout libraries. Based upon validated techniques published in the literature, the Sanger CRISPR libraries will put your lab at the forefront of the race to make the next big discovery.
アプリケーション
Functional Genomics/Screening /Target Validation
Sanger Arrayed Whole Genome Lentiviral CRISPR Library has been used for Functional Genomics/Screening /Target Validation.
特徴および利点
- Vector: U6-gRNA/PGK-Puro-2A-BFP (gRNA only)
- Simplify the workflow with puromycin selection
- Illuminate CRISPR-expressing cells with BFP
- Flip the expression components into and out of the genome using transposase
Additional Features
- Better, not bigger: Two optimized clones per human gene reduces the time, cost, and scale of screening experiments
- Ready-to-screen: Clones are arrayed in a robotics-friendly 96-well format for high throughput screening; requires plasmid preparation to transfect cells or lentivirus preparation to transduce cells
- Collaborative: Real-time, library validation continues
For detailed information on the Sanger library, click here
包装
96-well plates
構成
Bacteria glycerol stock of gRNA-only 3rd generation lenti-based plasmid vector. 2 knockout clones for every human protein-coding gene. Nearly 40,000 sequence confirmed clones
Request a Quote or More Information
Request a Quote or More Information
物理的形状
50 μl of bacterial glycerol stocks, 450×96-well plates
その他情報
This product is for R&D use only, not for drug, household, or other uses. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL-2) organisms in laboratory handling. Follow all published RGL-2 guidelines for laboratory handling and waste decontamination.
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保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
カルタヘナ法
カルタヘナ法
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries
Scientific Reports (2017)
資料
Genome-wide screening with optimized gRNAs per gene ensures specific and efficient knockout, controlling time and cost.
プロトコル
Learn about Sanger Sequencing steps or the chain termination method and how DNA sequencing works and how to read Sanger Sequencing results accurately for your research.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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