M4812

抗ミオシンVa (LF-18) ウサギ宿主抗体

~0.4 mg/mL, affinity isolated antibody, buffered aqueous solution

• 別名: Anti-GS1, Anti-MYH12, Anti-MYO5, Anti-MYR12
• MDL番号: MFCD03455613
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen 190 kDa
• 化学種の反応性: chicken, rat
• 強化検証: independent; Learn more about Antibody Enhanced Validation
• 濃度: ~0.4 mg/mL
• テクニック: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using rat and chicken cerebellum sections; microarray: suitable; western blot: 1:1,000 using a rat brain extract
• UniProtアクセッション番号: Q02440
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... MYO5A(4644) ; mouse ... Myo5a(17918) ; rat ... Myo5a(25017)

詳細

詳細

Anti-myosin Va (LF-18) recognizes myosin Va (190kDa). Myosin 5a is encoded by the gene mapped to human chromosome 15q21.2. The encoded protein is a member of the unconventional myosin family. It is a two-headed motor containing two identical heavy chains and multiple light chains. Myosin Va is widely expressed, but at high levels in the brain, testes and melanocytes.

免疫原

ニワトリミオシンVaのC末端付近に位置するアミノ酸配列1782-1799に相当する合成ペプチド(N末端リジン付加)のKLH結合体。この配列はヒト、マウス、ラットで一致しています。

アプリケーション

Anti-Myosin Va (LF-18) antibody produced in rabbit has been used in:

• Western blot.
• In situ proximity ligation assay (PLA).
• Glutathione S-transferase (GST)-spinophilin co-sedimentation assay.

生物化学的／生理学的作用

Myosin 5a is an actin-based molecular motor responsible for transport of secretory granules in various cell systems, including insulin granule. Additionally, it also mediates the transport of cargo such as cell-surface receptors, pigment and organelles like endoplasmic reticulum to the plasma membrane. The encoded protein facilitates pigmentation and synaptic activity in the central nervous system. Genetic variations in the gene are associated with the development of immunological and neurological diseases, including Griscelli syndrome type 1 and Elejalde syndrome.

物理的形状

0.01M PBS溶液 (1%BSA, 15mMアジ化ナトリウム含有)。

保管および安定性

For continuous use, store at 2-8°C for up to one month. For prolonged storage, freeze in working aliquots at -20°C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

その他情報

In order to obtain best results in different techniques and preparations we recommend determining optimal working dilutions by titration test.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable