LYSC9000

Lys-c (lysyl-endopeptidase), Active

from Achromobacter lyticus, recombinant, expressed in E. coli (206-473aa), tag-free, solution

• 別名: Lys-c (lysyl-endopeptidase), Active, API, Lysyl endopeptidase, Protease I
• Enzyme Commission number: 3.4.21.50 (BRENDA, IUBMB)
• UNSPSCコード: 12352204
• NACRES: NA.54

特性

• 由来生物: Achromobacter lyticus
• 品質水準: 200
• リコンビナント: expressed in E. coli (206-473aa)
• グレード: Proteomics Grade
• 形状: ready-to-use solution
• 濃度: 1 mg/mL
• 最適pH: 8.5
• pH範囲: 9.0-9.5(catalytic)
• UniProtアクセッション番号: P15636
• 輸送温度: dry ice
• 保管温度: −20°C

詳細

詳細

Recombinant tag-free Achromobacter lyticus Lys-c (lysyl-endopeptidase) (206-473aa) was expressed in E.coli cells.

Overview
Lysyl-endopeptidase (Lys-c) was isolated from the Gram-negative soil bacterium Achromobacter lyticus by Msaki et al. The protein hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues making it an important tool for enzymatic protein sequencing and Lys-X compound synthesis.

特異性

This enzyme hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues, at a catalytic pH range of 9.0-9.5, catalytic temperature range of 30-37 °C.

アプリケーション

The enzyme functions optimally between 30-37 °C and suffers from degradation when subjected to temperatures above 50 °C. Lysyl-endopeptidase retains complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30 °C.

包装

1mg/ml in Plastic

調製ノート

Catalytic pH range 9.0-9.5. Catalytic temperature range 30-37 °C.

保管および安定性

Store product at -20°C for up to one year. Avoid freeze/thaw cycles.

その他情報

For R&D only.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable