L5668

リポポリサッカリド 大腸菌0127:B8由来

Ready Made solution, 1 mg/mL

• 別名: LPS
• MDL番号: MFCD00212998
• UNSPSCコード: 12352201
• NACRES: NA.25

特性

• 由来生物: Escherichia coli (O127:B8)
• 品質水準: 200
• 形状: buffered aqueous solution
• 濃度: 1 mg/mL
• 色: colorless to faint yellow
• 溶解性: water: soluble
• 輸送温度: wet ice
• 保管温度: 2-8°C

詳細

詳細

This product is a 0.2 um filtered aqueous solution containing 1 mg/mL of lipopolysaccharides (LPS) from E. coli serotype O127:B8. This LPS serotype has been used in studies of septic shock, to induce neutrophils, to induce NOS in rat brain and induce PAF synthesis in rat glomerular mesangial cells.

アプリケーション

リポポリサッカリド（LPS）は、グラム陰性菌細胞壁の特徴的な構成要素です。LPS及びそのリピドA部分は、一般的な病原体関連分子パターン（PAMP）を認識するToll様受容体タンパク質ファミリーのメンバーであるToll様受容体4（TLR4）により自然免疫系細胞を刺激します。

生物化学的／生理学的作用

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

調製ノート

The LPS used to prepare the solution have been phenol-extracted and chromatographically purified by gel filtration. Lipopolysaccharides prepared by phenol extraction contain up to 60% RNA and less than 1% protein. Subsequent purification by gel filtration chromatography removes much of protein present in the phenol-extracted LPS, but leaves a product that still contains 10-20% nucleic acids. Further purification using ion exchange chromatography yields an LPS product which contains <1% protein and <1% RNA.

その他情報

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)