I2519

モノクローナル抗インターロイキン8抗体 マウス宿主抗体

clone 6217, purified immunoglobulin, lyophilized powder

• 別名: 抗CXCL8抗体, 抗IL-8抗体
• MDL番号: MFCD00212220
• UNSPSCコード: 51111800
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 結合体: unconjugated
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 6217, monoclonal
• フォーム: lyophilized powder
• 交差性: human
• テクニック: capture ELISA: suitable; flow cytometry: suitable; immunocytochemistry: 25 μg/mL using using calcium ionophore- and PMA-stimulated human peripheral blood mononuclear cells; neutralization: suitable; western blot: 1-2 μg/mL
• アイソタイプ: IgG1
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... IL8(3576)

詳細

詳細

Interleukin-8 or CXCL8 (IL-8) is a proinflammatory chemokine that belongs to the C-X-C family. Monocytes and macrophages are the primary sources of IL-8 but it is also secreted by most nucleated cells. IL-8 binds to two G protein-coupled receptors, CXCR1 and CXCR2 and leads to the downstream activation of PI3K, MAPK, PKC, STAT3 and FAK pathways. IL-8 is responsible for the recruitment monocytes and neutrophils that results in a prolonged acute inflammation. The expression of IL-8 is influenced by inflammatory signals, chemical and environmental stress and hormones. Deregulation of IL-8 signaling has been observed in atherosclerosis, cardiovascular disease and solid tumors of bladder, prostate, ovary, glial cells and pancreas. Monoclonal Anti-Interleukin-8 antibody recognizes human IL-8. By immunoblotting, the antibody shows 100% cross reactivity with recombinant porcine IL-8, and no cross reactivity with recombinant rat CINC-2α.

免疫原

E. coli発現リコンビナントヒトIL-8の精製物。

アプリケーション

Monoclonal Anti-Interleukin-8 antibody may be used for neutralization assays; ND50 is 0.08-0.4 μg/ml. The antibody may be used for immunoblotting at a working concentration of 1-2 μg/ml. For ELISA a working concentration of 0.5 μg/ml may be used. Antibody concentration of 25 μg/ml may be used for immunocytochemistry using stimulated human peripheral blood mononuclear cells. For flow cytometry, 10 μL of 25 μg/ml stock antibody solution may be used for 1-5 X 10⁵ cells.

物理的形状

凍結乾燥品 (PBS溶液, 5%トレハロ-ス含有, 0.2μmフィルタ-滅菌済み)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)