I17003

インターロイキン-4 ヒト

IL-4, recombinant, expressed in HEK 293 cells, suitable for cell culture, endotoxin tested

• 別名: IL-4
• MDL番号: MFCD00151705
• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• リコンビナント: expressed in HEK 293 cells
• 品質水準: 200
• アッセイ: ≥98% (SDS-PAGE)
• 有効性: ≤1 ng/mL ED₅₀
• 分子量: 14.9 kDa (glycosylated)
• テクニック: cell culture | mammalian: suitable
• 適合性: endotoxin tested
• 保管温度: −20°C

詳細

詳細

Recombinant human Interleukin-4 (IL-4) is expressed in human 293 cells as a glycoprotein with a calculated moleculaer mass of 14.9 kDa. This protein is manufactured in human cells using an all-human production system, with full chemically defined ingredients and with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports better stability of the protein in culture.

生物化学的／生理学的作用

インタ-ロイキン-4は、免疫担当細胞の増殖と分化に対して著しい効果を示すリンホカインです。IL-4 は、B細胞刺激因子-1(BSF-1)、T細胞増殖因子-2(TCGF-2)、肥満細胞増殖因子(MCGF-2)としても知られています。VEGF誘導血管形成とbFGF誘導血管形成を阻害します。IL-4は、活性化T細胞のサブセットにより分泌される糖タンパク質複合体です。ヒトIL-4とマウスIL-4のアミノ酸配列相同性は50%ですが、生物学的作用は種特異的です。

IL-4 is a protein that has been shown to regulate a wide spectrum of functions in B cells, T cells, monocytes/macrophages and other haematopoietic and non-haematopoietic cells. Among T cell clones, IL-4 is produced by Th0 and Th2 cells, but not Th1 cells, and this has now been demonstrated both in mice and in humans. IL-4 blocks the production of proinflammatory cytokines such IL-6, TNFα, and M-CSF and improves the differentiation of immature DCs from CD34+ progenitors when added during the differentiation period (day 6 to day 12).

シーケンス

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物理的形状

Supplied as a lyophilized powder containing phosphate buffered saline.

アナリシスノート

The biological activity of recombinant human IL-4 was tested in culture by measuring its ability to stimulate proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable