HPA008216

抗SEC23B ウサギ宿主抗体

Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

• 別名: 抗SEC23関連タンパク質B抗体 ウサギ宿主抗体, 抗タンパク質輸送タンパク質Sec23B抗体 ウサギ宿主抗体
• UNSPSCコード: 12352203
• Human Protein Atlas Number: HPA008216
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 製品種目: Prestige Antibodies^® Powered by Atlas Antibodies
• フォーム: buffered aqueous glycerol solution
• 交差性: rat, mouse, human
• テクニック: immunofluorescence: 0.25-2 μg/mL; immunohistochemistry: 1:200-1:500
• 免疫原配列: MVQVHELSCEGISKSYVFRGTKDLTAKQIQDMLGLTKPAMPMQQARPAQPQEHPFASSRFLQPVHKIDMNLTDLLGELQRDPWPVTQGKRPLRSTGV
• UniProtアクセッション番号: Q15437
• 輸送温度: wet ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... SEC23B(10483)

詳細

詳細

The gene encoding protein transport protein Sec23 homolog B (SEC23B) is about 54kb long and located on the chromosome 20p11.23. It is composed of five domains: zinc finger domain, trunk domain, β-sheet domain, helical domain and gelsolin domain.

免疫原

タンパク質輸送タンパク質Sec23BのPrEST(recombinant protein epitope signature tag)抗原リコンビナントタンパク質。

アプリケーション

Prestige抗体^®は、Human Proteome Resource(HPR)プロジェクト(www.proteinatlas.org)によって開発・実証されたAtlas抗体です。抗体はすべて、数百の正常組織・疾病組織に対する免疫組織染色試験を行っています。これらの染色画像はHuman Protein Atlas(HPA)サイトで[Image Gallery]リンクをクリックするとご覧いただけます。さらに、ほとんどのPrestige抗体はプロテインアレイおよびウェスタンブロッティングの試験を行っています。試験のプロトコールおよびPrestige抗体、HPAに関する情報はsigma.com/prestigeをご覧ください。

生物化学的／生理学的作用

Protein transport protein Sec23 homolog B (SEC23B) is part of the coat protein complex II (COPII) which is required by proteins to be transported out of the endoplasmic reticulum (ER). It is a secretion-associated RAS-related protein 1 (SAR1) GTPase-activating protein and also plays a role in cargo recognition. Conversion of SAR1-GTP to SAR1-GDP causes its dissociation from the COPII coat. SAR1-bound SEC23B interacts with the C terminus of SEC31, creating a link between the outer-coat SEC13–SEC31 complex and the inner-coat SAR1–SEC23B–SEC24 complex. When it interacts with the outer coat, the GAP activity is stimulated and this accelerates the GTP hydrolysis of SAR1. SAR1 gets released and SEC23B interacts with the trappin protein transglutaminase (TRAPPI) complex subunits like dynactin which are all involved in vesicle tethering, fusion and transportation along microtubules. Finally, SEC23 is phosphorylated and this leads to the fusion of COPII vesicles with the target membrane.

特徴および利点

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

関連事項

Corresponding Antigen APREST86883

物理的形状

PBS溶液 (pH 7.2, 40%グリセロ-ル, 0.02%アジ化ナトリウム含有)

法的情報

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)