HPA005719

Anti-USP33 antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

• 別名: Anti-KIAA1097, Anti-VDU1, Anti-ubiquitin specific peptidase 33
• UNSPSCコード: 12352203
• Human Protein Atlas Number: HPA005719
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous glycerol solution
• 交差性: human
• テクニック: immunoblotting: 0.04-0.4 μg/mL; immunofluorescence: 0.25-2 μg/mL; immunohistochemistry: 1:500-1:1000
• 免疫原配列: ESQVDHSTIHSQETKHYLTVNLTTLRVWCYACSKEVFLDRKLGTQPSLPHVRQPHQIQENSVQDFKIPSNTTLKTPLVAVFDDLDIEADEEDELRARGLTGLKNIGNTCYMNAALQALS
• UniProtアクセッション番号: Q8TEY7
• 輸送温度: wet ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... USP33(23032)

詳細

詳細

Ubiquitin specific peptidase 33 (USP33) belongs to the ubiquitin-specific protease (USP) subclass. It is localized to the cytoplasm, including the endoplasmic reticulum. A short isoform of USP33 is present in the Golgi apparatus.

免疫原

ユビキチン特異的ペプチダーゼ33のPrEST(protein epitope signature tag)抗原リコンビナントタンパク質。

アプリケーション

Prestige抗体^®は、Human Proteome Resource(HPR)プロジェクト (www.proteinatlas.org)によって開発・実証されたAtlas抗体です。抗体はすべて、数百の正常組織・疾病組織に対する免疫組織染色試験を行っています。これらの染色画像はHuman Protein Atlas(HPA)サイトで[Image Gallery]リンクをクリックするとご覧いただけます。さらに、ほとんどのPrestige抗体はプロテインアレイおよびウェスタンブロッティングの試験を行っています。試験の プロトコール およびPrestige抗体、HPAに関する情報は sigma.com/prestigeをご覧ください。

生物化学的／生理学的作用

Agonist-induced ubiquitination of the β2 adrenergic receptor (β2AR) is an important post-translational modification to sort internalized receptors to the lysosomes for degradation. This ubiquitination is reversed by ubiquitin specific peptidase 3 (USP33), hence inhibiting lysosomal trafficking, promoting receptor recycling from the late-endosomal compartments and re-sensitization of recycled receptors at the cell surface. Dissociation of the bound USP33 from the β2AR immediately after agonist stimulation and re-association on prolonged agonist treatment, allows receptors to first become ubiquitinated and then deubiquitinated. Thus, this acts as a ‘trip switch′ between degradative and recycling pathways at the late-endosomal compartments. USP33 also binds to β-arrestin2 and leads to the deubiquitination of β-arrestins. Along with Mdm2, it functions to favor the stability receptor β-arrestin complex, thus regulating its longevity and subcellular localization.

特徴および利点

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

関連事項

Corresponding Antigen APREST70778

物理的形状

PBS溶液(pH 7.2, 40%グリセロールおよび0.02%アジ化ナトリウム含有)。

法的情報

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable