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Merck

GERPN2236

ECL Prime Western Blotting Detection Reagent

Cytiva RPN2236

別名:

chemiluminescent detection reagent, Western blotting detection reagent

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About This Item

UNSPSCコード:
41105332
NACRES:
NA.32

包装

pkg of 1 kit

メーカー/製品名

Cytiva RPN2236

関連するカテゴリー

詳細

Amersham ECL Prime is a highly sensitive reagent, characterized by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. The high intensity of the emitted signals means that proteins can be detected using highly diluted primary and secondary antibodies, contributing to low background and reducing consumption of reagents.

Amersham ECL Prime consolidates and builds on the benefits of Amersham ECL Plus and Amersham ECL Advance to deliver a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents.

アプリケーション

ECL Prime Western blotting detection reagent has been used to detect chemiluminescent signals during Western blotting.

特徴および利点

  • Compatible with Rainbow Molecular Weight Markers and Amersham ECL DualVue Western Blotting Markers.
  • High signal intensity and sensitivity allows the use of highly diluted primary and secondary antibodies with no reduction in sensitivity.
  • Stable signal allows multiple exposures and makes the reagent suitable for large experimental series, allowing convenient handling time between the end of the experiment and detection.
  • Optimized for use with Amersham Hybond-P® (PVDF) membranes and compatible with Amersham Hybond-ECL (nitrocellulose) membranes.
  • Optimized for imaging with ImageQuant LAS series (CCD-based imaging) and compatible with Amersham Hyperfilm.

構成

The following components are included in the Amersham ECL Prime detection reagent kit.
  • RPN2236 Solution A: Luminol solution, 3 × 50 ml Solution B: Peroxide solution, 3 × 50 ml sufficient for 3000 cm2 membrane

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

Amersham is a trademark of Cytiva
ECL is a trademark of Cytiva
ECL DualView is a trademark of Amersham
Hybond is a registered trademark of Cytiva
Hyperfilm is a trademark of Cytiva

保管分類コード

12 - Non Combustible Liquids

引火点(°F)

Not applicable

引火点(℃)

Not applicable


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Registered report: Melanoma genome sequencing reveals frequent PREX2 mutations
Denise Chroscinski
eLife, 3 (2014)

資料

Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.

分子生物学者がメンブレン(膜)に結合したタンパク質または核酸のサンプルを検出するために使用されるさまざまな方法のプロトコルと背景説明

This page describes troubleshooting strategies in the detection of GST-tagged proteins.

プロトコル

This page describes chemiluminescence detection of GST-tagged proteins with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select from Cytiva.

This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using Cytiva products.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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