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Merck

GE29-0485-81

HiTrap® Protein G HP

Cytiva 29-0485-81, pack of 1 mL

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About This Item

UNSPSCコード:
23151816
NACRES:
NA.56

ligand

recombinant protein G lacking albumin-binding region

包装

pack of 1 mL

メーカー/製品名

Cytiva 29-0485-81

利用可能性

not available in North America

保管条件

(20% Ehtanol)
(20% Ethanol)

パラメーター

flow rate
42 psi

ベッドサイズ

7 mm × 25 mm

ベッド容積

1 mL

カラム内径

7 mm

マトリックス

highly cross-linked 6% agarose

平均部品サイズ

34 μm

cleaning

2-9

working range

3-9

キャパシティ

>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
25 mg binding capacity(human IgG/column)

保管温度

2-8°C

詳細

HiTrap Protein G HP columns are prepacked with Protein G Sepharose High Performance for fast, easy separations of IgG from different species and subclasses.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

特徴および利点

High binding capacity of 25 mg IgG/ml medium and fast binding kinetics.
34 μm bead size for high performance, high-resolution purifications.
Binds broad range of different species and subclasses of IgG.
Simple and proven method giving reproducible results.
Designed for use with syringe, peristalic pump, or chromatography system.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

その他情報

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法的情報

HiTrap is a registered trademark of Cytiva

ピクトグラム

Flame

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Philipp A Kronenberg et al.
Frontiers in cellular and infection microbiology, 13, 1162530-1162530 (2023-04-04)
Alveolar (AE) and cystic echinococcosis (CE) are severe parasitic zoonoses caused by the larval stages of Echinococcus multilocularis and E. granulosus sensu lato, respectively. A panel of 7 monoclonal antibodies (mAbs) was selected against major diagnostic epitopes of both species.

資料

Designing purification methods for antibodies ensures quality and quantity adequacy for research and industrial purposes.

Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic fluid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.

This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.

すべて表示

プロトコル

This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap® Protein G HP from Cytiva for the capture step.

General instructions for affinity purification using HiTrap columns from Cytiva.

This page shows how to prepare samples for purification with affinity chromatography.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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