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ligand
quaternary amine
詳細
Ion Exchanger Type (value)
包装
pkg of 7 ea
メーカー/製品名
Cytiva 17-6002-33
パラメーター
42 psi
ベッドサイズ
7 mm × 25 mm
ベッド容積
1 mL
カラム内径
7 mm
Matrix
6% cross-linked agarose
粒径
45-165 μm
平均直径
90 μm
cleaning
2-14
動作範囲
2-12
適合性
suitable for bioprocess medium
関連するカテゴリー
詳細
Q, SP, DEAE, and CM Sepharose™ Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose™ 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.Q and SP Sepharose™ XL media have long chains of dextran coupled to a robust, 6% highly cross-linked agarose matrix. The dextran chains increase the exposure of the Q or SP charged groups, which results in very high loading capacities. The active end of the charged group is the same for DEAE Sepharose Fast Flow and ANX Sepharose™ Fast Flow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose Fast Flow has a diethylaminoethyl-group bound to the agarose whilst ANX Sepharose™ 4 Fast Flow (high sub) has a diethylaminopropyl group attached. After choosing the optimal medium, prepacked columns and bulk media are available for larger scale preparative work.
特徴および利点
- Fast and easy selection with seven different ion-exchange ligands on Sepharose™ Fast Flow and Sepharose™ XL, prepacked in 1 mL HiTrap® columns.
- Offers a fast, easy and convenient way screen for the optimal media for a given application .
- Excellent for optimization studies; buffer composition, pH, flow rate, sample loading, and elution scheme can be optimized with small sample quantities prior to scale-up.
- Designed for use with a syringe, peristaltic pump, or chromatography system such as AKTA design.
保管および安定性
アナリシスノート
法的情報
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
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資料
This page provides information about the principles and standard conditions for different purification techniques for the purification of multiprotein complexes with from Cytiva.
This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
プロトコル
This page covers the principles and methods of chromatofocusing, a chromatography technique that separates proteins according to differences in their isoelectric point (pI).
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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