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Merck

GE17-6002-33

HiTrap® IEX Selection Kit

Cytiva 17-6002-33

別名:

IEX Selection Kit

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About This Item

UNSPSCコード:
23151816
NACRES:
NA.56

ligand

quaternary amine

詳細

Ion Exchanger Type (value)

包装

pkg of 7 ea

メーカー/製品名

Cytiva 17-6002-33

パラメーター

42 psi

ベッドサイズ

7 mm × 25 mm

ベッド容積

1 mL

カラム内径

7 mm

Matrix

6% cross-linked agarose

粒径

45-165 μm

平均直径

90 μm

cleaning

2-14

動作範囲

2-12

適合性

suitable for bioprocess medium

関連するカテゴリー

詳細

HiTrap® IEX Selection Kit consists of seven HiTrap® columns, prepacked with different Sepharose Fast Flow ion exchange chromatography media for screening of the most appropriate media and conditions to use for specific application and development work.

Q, SP, DEAE, and CM Sepharose Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.Q and SP Sepharose XL media have long chains of dextran coupled to a robust, 6% highly cross-linked agarose matrix. The dextran chains increase the exposure of the Q or SP charged groups, which results in very high loading capacities. The active end of the charged group is the same for DEAE Sepharose Fast Flow and ANX Sepharose Fast Flow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose Fast Flow has a diethylaminoethyl-group bound to the agarose whilst ANX Sepharose 4 Fast Flow (high sub) has a diethylaminopropyl group attached. After choosing the optimal medium, prepacked columns and bulk media are available for larger scale preparative work.

特徴および利点

  • Fast and easy selection with seven different ion-exchange ligands on Sepharose Fast Flow and Sepharose XL, prepacked in 1 mL HiTrap® columns.
  • Offers a fast, easy and convenient way screen for the optimal media for a given application .
  • Excellent for optimization studies; buffer composition, pH, flow rate, sample loading, and elution scheme can be optimized with small sample quantities prior to scale-up.
  • Designed for use with a syringe, peristaltic pump, or chromatography system such as AKTA design.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol (Q, DEAE, ANX,CM), 20% Ethanol, 0.2 M Sodium Acetate (SP))
Store at 4 to 30 °C (20% Ethanol)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

ピクトグラム

Flame

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


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資料

This page provides information about the principles and standard conditions for different purification techniques for the purification of multiprotein complexes with from Cytiva.

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

すべて表示

プロトコル

This page covers the principles and methods of chromatofocusing, a chromatography technique that separates proteins according to differences in their isoelectric point (pI).

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

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