GE17-5478-51

HiTrap^® Protein L

Cytiva 17-5478-51, pack of 5 × 1 mL

• 別名: HiTrap Chromatography Column, Protein L Column
• UNSPSCコード: 23151816
• NACRES: NA.56

特性

• ligand: recombinant protein L (E. coli), mammalian free
• 包装: pack of 5 × 1 mL
• メーカー／製品名: Cytiva 17-5478-51
• 保管条件: (20% Ehtanol)
• パラメーター: 5 bar
• カラム内径: 7 mm
• マトリックス: rigid, highly cross-linked agarose
• 平均部品サイズ: 85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
• working range: 2-10(pH interval where the medium can be operated without significant change in function.)
• 適合性: suitable for bioprocess medium
• 保管温度: 2-8°C

詳細

詳細

HiTrap™ Protein L columns are prepacked with Capto™ L affinity chromatography resin for purification of antibody fragments containing the kappa light chain. This resin combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which has a strong affinity to the variable region of antibody kappa light chains.

HiTrap^® Protein L columns are packed with Capto^™ L affinity chromatography resin. It facilitates the purification of antibody fragments comprising the κ light chain. Capto^™ L resin combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which binds to the variable region of antibody κ light chains with strong affinity. This resin is an effective capture platform for antibody fragments having κ light chains due to its strong binding capacity and affinity to a wide spectrum of antibody fragments.

アプリケーション

HiTrap^® Protein L may be used to characterize human colorectal cancer MDR1/P-gp Fab and for the purification optimization of a single-chain variable fragment (scFv) against type 1 insulin-like growth factor receptor (IGF-1R). It may also be used for the purification of antigen-binding fragments (Fabs) and domain antibodies (dAbs).

特徴および利点

• Captures a wide range of antibody fragments
• Allows a platform approach for the purification of antibody fragment
• Offers low ligand leakage, high binding capacity, and selectivity for a broad range of antibody fragments
• Reduces process time and amount of resin
• Suitable for process development and small-scale purification
• HiTrap^® format easily connects to a syringe, peristaltic pump, or chromatographic equipment, such as an ÄKTA^® system.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

Capto is a trademark of Cytiva

HiTrap is a registered trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

安全性情報

• ピクトグラム: GHS02
• シグナルワード: Warning
• 危険有害性情報: H226
• 注意書き: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 保管分類コード: 3 - Flammable liquids