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Merck

GE17-0921-08

IMAC Sepharose 6 Fast Flow

Cytiva 17-0921-08, pack of 100 mL

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About This Item

UNSPSCコード:
41106500
NACRES:
NA.56

包装

pack of 100 mL

メーカー/製品名

Cytiva 17-0921-08

マトリックス

6% cross-linked agarose

粒径

45-165 μm

平均直径

90 μm

cleaning in place

2-14(Medium uncharged with metal ions.)

working range

3-12

キャパシティ

~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)

適合性

suitable for bioprocess medium

関連するカテゴリー

詳細

IMAC Sepharose 6 Fast Flow is a BioProcess IMAC medium, designed for purifying proteins and peptides that have an affinity for metal ions, such as histidine-tagged proteins but also untagged recombinant or native proteins.

IMAC Sepharose 6 Fast Flow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.

IMAC Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix. IMAC Sepharose 6 Fast Flow is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target protein.

The coupling chemistry and the novel ligand is designed to give minimal ligand leakage while providing high capacity.

IMAC Sepharose 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, IMAC Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

特徴および利点

  • Uncharged IMAC resin allowing possibility to charge with your metal ion of choice for optimized selectivity
  • Convenient purification of histidine-tagged proteins, also when Ni2+ is not the best choice of metal ion
  • As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
  • Available in the convenient and time-saving prepacked HiTrap® and HiPrep formats.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
HiPrep is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

ピクトグラム

Flame

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

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資料

Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

How to optimize purification of histidine-tagged proteins using Cytiva products.

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

プロトコル

This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from Cytiva.

This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using Cytiva products.

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

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