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包装
pack of 100 mL
メーカー/製品名
Cytiva 17-0921-08
マトリックス
6% cross-linked agarose
粒径
45-165 μm
平均直径
90 μm
cleaning in place
2-14(Medium uncharged with metal ions.)
working range
3-12
キャパシティ
~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)
適合性
suitable for bioprocess medium
関連するカテゴリー
詳細
IMAC Sepharose™ 6 Fast Flow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.
IMAC Sepharose™ 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix. IMAC Sepharose™ 6 Fast Flow is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target protein.
The coupling chemistry and the novel ligand is designed to give minimal ligand leakage while providing high capacity.
IMAC Sepharose™ 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, IMAC Sepharose™ 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
特徴および利点
- Uncharged IMAC resin allowing possibility to charge with your metal ion of choice for optimized selectivity
- Convenient purification of histidine-tagged proteins, also when Ni2+ is not the best choice of metal ion
- As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
- Available in the convenient and time-saving prepacked HiTrap® and HiPrep™ formats.
保管および安定性
アナリシスノート
法的情報
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
資料
Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.
How to optimize purification of histidine-tagged proteins using Cytiva products.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.
プロトコル
This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from Cytiva.
This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using Cytiva products.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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