G6919

Anti-G9a Methyltransferase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• 別名: 抗BAT8抗体
• MDL番号: MFCD06411814
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 化学種の反応性: human
• テクニック: immunoprecipitation (IP): 1-2 μg using 293-T cell extracts; microarray: suitable; western blot: 1-2 μg/mL using nuclear extracts of 293-T cells
• UniProtアクセッション番号: Q96KQ7
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... EHMT2(10919) ; mouse ... Ehmt2(110147)

詳細

詳細

G9a (also known as HLA-B-associated transcript 8 (BAT8), G9a/NG36, or NG36) was originally characterized as a molecule encoded by a gene mapped in the class III region of the human major histocompatibility complex locus. G9a as well as EuHTM1 (Eu-HMTase) were found to be the components of the multimeric protein complex E2F-6.

特異性

G9aメチルトランスフェラ-ゼの2種類のアイソフォ-ム(約180 kDaおよび160 kDa)を認識します。

免疫原

ヒトG9aのアミノ酸1193~1210に相当する合成ペプチド(N末端システイン付加)のKLH結合体。この配列は、マウスの配列と3アミノ酸異なっています。

アプリケーション

Anti-G9a Methyltransferase antibody produced in rabbit has been used in:

• immunoblotting
• immunoprecipitation
• immunohistochemical analysis (IHC)

生物化学的／生理学的作用

In vivo, G9a is essential for early embryonic development and plays a dominant role in H3-K9 methylation of euchromatin. G9a was found to be a critical component of the CtBP and E2F-6 transcriptional repressor complexes. G9a was found to be the enzyme responsible for mono and dimethylation within silent euchromatin.

G9a is a lysine-preferring histone methyltransferase (HMTase) containing a SET domain. It has 10 to 20-fold stronger in vitro HMTase activity towards histone H3-Lys9 (H3-K9) when compared to Suv39H1, another member of the same family of HMTases. It also methylates Lys27 of histone H3. Suv39 h1 methylates only lysine 9. It is capable of mono and dimethylation within silent euchromatin, whereas Suv39h1 trimethylates at pericentric heterochromatin. G9a is an important factor in the early embryonic development and plays a dominant role in H3-K9 methylation of euchromatin in vivo. It also forms a crucial component of the CtBP and E2F-6 transcriptional repressor complexes.

物理的形状

0.01 M PBS溶液(pH 7.4, 15 mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable