G3548

Anti-GADD45A (12-25) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• 別名: Anti-DDIT1, Anti-GADD45, Anti-Growth arrest and DNA-damage-inducible protein GADD45 α
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: IgG fraction of antiserum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~18 kDa
• 化学種の反応性: human
• テクニック: western blot: 1:500-1:2,000
• UniProtアクセッション番号: P24522
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... GADD45A(1647)

詳細

詳細

Growth arrest and DNA damage inducible α It is expressed at low levels in testis, but, at moderate level in liver, brain, heart and spleen. GADD45A shows high expression in kidney and skeletal muscle.

免疫原

synthetic peptide corresponding to amino acids 12-25 of human GADD45A

アプリケーション

Yale Center for High Throughput Cell Biology IF検査済み抗体 各抗体は、Yale HTCB IFプロトコルールを用いてHUVEC細胞に対する免疫蛍光法（IF）によって検査されます。Sigma Life ScienceおよびYale Center for High Throughput Cell Biology のパートナーシップの詳細については、sigma.com/htcb-ifをご覧ください。

Anti-GADD45A (12-25) antibody produced in rabbit has been used in immunocytochemistry.

生物化学的／生理学的作用

GADD45A (growth arrest and DNA-damage-inducible, alpha) gene encodes a protein that belongs to a group of genes that are expressed under stressful growth arrest conditions and upon treatment with DNA-damaging agents such as methylmethane sulfonate and UV radiation. The protein mediates activation of the p38/JNK pathway via MTK1/MEKK4 kinase upon environmental stress. It inhibits entry of cells into S phase by activating DNA excision repair in vitro. It plays a crucial role in cell cycle checkpoints, DNA repair, and genome integrity maintenance.

Growth arrest and DNA damage inducible α (GADD45A) miRNAs are differentially expressed in osteosarcoma. It interacts with proliferating cell nuclear antigen (PCNA), aurora A kinase and histones. GADD45A improves the cytotoxic effect of the drug temozolomide (TMZ) in glioblastoma multiforme (GBM).

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable