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由来生物
mouse
結合体
unconjugated
抗体製品の状態
ascites fluid
抗体製品タイプ
primary antibodies
クローン
1B10, monoclonal
分子量
antigen 43-72-80 kDa
含みます
15 mM sodium azide
化学種の反応性
human
テクニック
complement-mediated cytotoxicity assay: 1:500
flow cytometry: suitable
immunohistochemistry (frozen sections): suitable
アイソタイプ
IgM
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
関連するカテゴリー
詳細
Monoclonal Anti-Human Fibroblast Surface Protein (mouse IgM isotype) is derived from the 1B10 hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with cultured human thymic fibroblasts. Human fibroblast surface protein is a fibroblast antigen that is expressed mainly on human synovial, foreskin and thymic fibroblasts and is absent on human epithelial cells and lymphocytes.
免疫原
培養ヒト胸腺繊維芽細胞
アプリケーション
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Monoclonal Anti-Fibroblast Surface Protein antibody produced in mouse has been used in:
- immunofluorescence
- flow cytometry
- immunohistochemistry
- immunoblotting
Monoclonal anti-fibroblast surface protein antibody can be used in flow cytometry of human fibroblasts and monocytes. It is also useful in immunoblotting and western blotting.
生物化学的／生理学的作用
Monoclonal Anti-Fibroblast Surface Protein antibody is also used to remove fibroblasts from human cultured cells by inhibiting fibroblasts adherence to the culture vessel or by cytotoxic effect in the presence of rabbit complement. In addition, apart from the functional significance of cells characteristics, the characterization of cell lines is important for correlation of cultures with tissue origin, identification of the lineage to which the cells belong or the precursor status of the cells. Monoclonal antibodies to fibroblast surface protein should provide a powerful tool for control of fibroblast growth in a variety of human cell culture systems, such as thymic epithelial (TE) cell cultures allowing the growth of highly enriched populations of the TE cells.
ターゲットの説明
この繊維芽細胞抗原は、ヒト滑膜、乳腺、包皮、胸腺の繊維芽細胞や悪性線維肉腫の組織で明らかにされています。抗原は、細胞膜とリソソ-ムの両方に結合しています。
アナリシスノート
補体を介した微量細胞傷害性試験での使用希釈率は1:500以上です。
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
nwg
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
F4771-VAR:
F4771-BULK:
F4771-.5ML:
F4771-.2ML:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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Yuki Sekino et al.
FEBS open bio (2021-06-12)
There are no human cancer cell lines of external auditory canal origin available for research use. This report describes the establishment of a culture condition for external auditory canal squamous cell carcinoma, derived from human tumor tissue. Successive squamous cell
M Olovsson et al.
Gynecologic and obstetric investigation, 49(3), 165-169 (2000-03-24)
To investigate the ability of human uterine myocytes to grow under anaerobic conditions for a prolonged time period. Cells were isolated from fundal myometrium and cultured until subconfluency. The cell type was confirmed by immunostaining for the smooth muscle cell-specific
Carcinoma-Associated Fibroblast-Like Differentiation of Human Mesenchymal Stem Cells
Mishra PJ, et al.
Cancer research, 68(11), 4331-4339 (2008)
Christian Hagel et al.
Neuropathology : official journal of the Japanese Society of Neuropathology, 32(4), 406-414 (2011-12-02)
The tumor suppressor disorder neurofibromatosis type 1 (NF1) is associated with development of multiple neurofibromas which may grow intraneurally as plexiform neurofibromas (PNF) or intracutaneously (CNF). Upon surgery neurofibromas may show prominent swelling hindering skin-edge approximation. To assess whether the
Pravin J Mishra et al.
International journal of translational science, 2016(1), 5-32 (2016-06-14)
We have previously demonstrated that human mesenchymal stem cells (hMSCs) migrate toward human keratinocytes as well as toward conditioned medium from cultured human keratinocytes (KCM) indicating that the hMSCs respond to signals from keratinocytes [1]. Using fluorescently labeled cells we
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