F1773

モノクロナール抗CD4

clone Q4120, purified from hybridoma cell culture

• 別名: Monoclonal Anti-CD4
• NACRES: NA.44
• UNSPSC Code: 12352203
• Conjugate: FITC conjugate
• Clone: Q4120, monoclonal
• Application: FACS
• Citations: 11

特性

• biological source: mouse
• Quality Segment: 200
• conjugate: FITC conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: Q4120, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 59 kDa
• species reactivity: human
• technique(s): flow cytometry: 10 μL using 1 × 10⁶ cells
• isotype: IgG1
• UniProt accession no.: P01730
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... CD4(920)

詳細

General description

CD4は、免疫グロブリン・ス-パ-ファミリ-に由来する単一鎖の膜貫通型糖タンパク質です。CD4は、ヘルパ-/インデュ-サ-T細胞サブセットをはじめ、大部分の胸腺髄質細胞、あるいはミクログリア細胞、樹状細胞やT細胞起源のある種の悪性腫瘍に発現しています。CD4は、MHCクラスII分子に結合し、p56^lckプロテインチロシンキナ-ゼと結合します。

Anti-CD4-FITC antibody, mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cell line NS-1 and splenocytes from Balb/c mice immunized with cluster of differentiation 4 (CD4)-transfected mouse T cell hybridoma, 3DT, followed by CD4⁺ human T cell CEM cells (leukemic lymphoblasts).

CD4, a glycoprotein, is predominantly expressed on the cell membrane of mature, thymus-derived (T) lymphocyte and also to some extent in the monocyte/macrophage lineage. It has a transmembrane domain, cytoplasmic region and an extracellular region of four tandem domain similar like members of Ig superfamily.

Immunogen

CD4をトランスフェクトしたマウスT細胞ハイブリド-マ3DT、続いてCD4+ヒトT細胞由来CEM細胞

Application

FITC Conjugated Monoclonal Anti-Human CD4 has been used for:

• identification, quantification and monitoring of helper/inducer T cells in peripheral blood, biological fluids, lymphoid organs, and other tissues
• analysis of T cell mediated cytotoxicity
• characterization of subtypes of T cell leukemias and lymphomas
• studies of T cells in health and disease
• flow cytometry

Biochem/physiol Actions

CD4を認識します。クロ-ン番号Q4120モノクロ-ナル抗体が認識するエピト-プは、アミノ酸1-183に位置しており、ホルマリン固定およびパラフィン包埋の影響をうけます。5th Workshop: code no. CD04.11

CD4⁺ T cells acts as a helper/inducer, in providing an activating signal to B cells. It induces T lymphocytes bearing the reciprocal CD8 marker to become cytotoxic/suppressor cells. It is also majorly associated with non-polymorphic region of MHC (major histocompatibility complex) antigen in interacting with targets bearing MHC class II molecules. Thus, it is called as an adhesion molecule. It also behaves as receptor for the GP 120 envelope glycoprotein of human immunodeficiency virus. The CD4 molecules is involved in the adhesion of T lymphocytes to target cells, thymic development, transmission of intracellular signals during T cell activation, and binding to polyclonal immunoglobulins.

Immunoregulatory T cell subset abnormalities in autoimmunity disorders, immunodeficiency diseases, graft versus host disease and following immunosuppressive therapy are often manifested as a change in CD4⁺/CD8⁺ ratio in peripheral blood T cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)