The stability of this Fibronectin solution upon further dilution has not been evaluated. At the prepared 1 mg/mL concentration, the solution is stable for at least 1 year from the date of shipment, when stored at 2-8°C. Please see the following links to review additional information regarding usage and handling of Fibronectin:
Coating protocol
https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/cell-growth-and-maintenance/fibronectin-product-protocols
General information
https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/mammalian-cell-culture/fibronectin
F1141
Bovine Fibronectin
from bovine plasma, liquid, suitable for cell culture
別名:
フィブロネクチン
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| あなたへ/カタログ番号 | 在庫状況 | 単価 |
|---|---|---|
1 mg | 在庫あり出荷可能from市川市大町368 | ￥32,300 |
2 mg | カートの在庫状況を確認する | ￥55,300 |
5 mg | 在庫あり出荷可能from市川市大町368 | ￥109,000 |
この商品について
Biological source:
bovine plasma
Form:
solution
Technique(s):
cell culture | mammalian: suitable
製品名
ウシ血漿フィブロネクチン, solution, sterile-filtered, BioReagent, suitable for cell culture
biological source
bovine plasma
Quality Segment
sterility
sterile-filtered
product line
BioReagent
form
solution
packaging
pkg of 1 mg, pkg of 2 mg, pkg of 5 mg
technique(s)
cell culture | mammalian: suitable
surface coverage
1‑5 μg/cm2
UniProt accession no.
binding specificity
Peptide Source: Collagen
, Peptide Source: Fibrinogen
shipped in
wet ice
storage temp.
2-8°C
Gene Information
bovine ... FN1(280794)
General description
フィブロネクチンは、細胞外マトリックス中の普遍的なタンパク質です。3種類の繰り返しモジュラーアミノ酸コンセンサス配列からなる、モザイクタンパク質です。フィブロネクチンは、高分子量糖タンパク質のグループに属します。細胞フィブロネクチンは、線維芽細胞によって発現され、血漿フィブロネクチンは、通常肝細胞によって遊離されます。
Application
ウシ血漿フィブロネクチン（FN）は、以下に使用されています：
- 吸着分析（ELISA、酵素結合免疫測定法）
- トランスウェル遊走
- フィブロネクチンアッセイ
Biochem/physiol Actions
フィブロネクチンは、細胞接着、細胞骨格形成、癌化、細胞遊走、ファゴサイトーシス、止血、および胚性分化に関与しています。
Analysis Note
タンパク質の均一性は、免疫電気泳動によって評価されています。
Other Notes
製品F1141、ウシ血漿由来フィブロネクチンは、0.05 M TBS、pH 7.5の溶液です。
1 of 1
当該品目 | |||
|---|---|---|---|
| sterility sterile-filtered | sterility sterile | sterility sterile | sterility sterile |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| biological source bovine plasma | biological source bovine plasma | biological source human plasma | biological source rat plasma |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form solution | form powder | form lyophilized powder | form powder |
| shipped in wet ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
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signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
保管分類
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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How long can I store the diluted solution (20µg/ml) in PBS?
1 回答-
役に立ちましたか？
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Can I freeze the aliquoted Fibronectin?
1 回答-
The recommended storage temperature for this product is 2-8°C. While freezer storage is not required, storing this solution in frozen aliquots at -20°C will not impact the integrity of the protein. Avoid repeated freeze/thaw cycles. The long term stability of this material when stored frozen has not been evaluated and may not result in extended shelf life.
役に立ちましたか？
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Hi, I bought a vial with 5mg of Fibronectin (F1141), but so far, I have not been able to find the concentration of this solution. Can you please help me with this info? Thanks a lot in advance!
1 回答-
The concentration of this product is 1 mg/mL. This information is reported in the product Certificate of Analysis. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/f1141#product-documentation役に立ちましたか？
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How long can I store fibronectin coated plates, etc?
1 回答-
Fibronectin coated cultureware can be stored for 2-4 weeks at 2-8 °C in a closed sterile container, or in sterile sealable bags.
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What is the procedure for coating culture surfaces with fibronectin?
1 回答-
To coat culture surfaces: 1. Dilute fibronectin in sterile balanced salt solution and coat the culture surface (1-5 μg/cm2) with a minimal volume. 2. Allow to air dry for at least 45 minutes at room temperature. Excess fibronectin may be removed by aspiration, but this is not necessary.
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
役に立ちましたか？
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How long can I store the fibronectin solution?
1 回答-
The product is good for at least a year at 2-8 °C if kept sterile.
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How do I choose which fibronectin product to use to coat culture surfaces for cell growth?
1 回答-
The species source for the fibronectin used when coating culture surfaces is not important. It is the presence of the RGD binding site that is present in all fibronectins that promotes binding.
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