This will fluctuate due to sample variability (sample collection, concentration, fragment length, sequence [GC content], storage before isolation, etc.). However, the sample is buffer exchanged into a standard storage buffer that is included in the kit (1X TE).
EC9999
GenElute™-E Single Spin Cap Puncher
For use with Genelute™-E kits. 1 cap puncher.
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別名:
GenElute™-E Cap Puncher
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この商品について
NACRES:
NA.52
UNSPSC Code:
41105600
現在、価格および在庫状況を閲覧できません。
usage
cap punching
feature
Intended use (For use with GenElute™-E Single Spin purification columns)
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
greener alternative category
storage temp.
room temp
General description
GenElute™-E purification kits provide a simple alternative for DNA purification. Compared with traditional methods, GenElute™-E offers:
Traditional silica-based, bind-wash-elute purification kits require multiple wash steps to remove impurities from the spin columns. These steps increase the risk of cross contamination, subject the DNA to centrifugation sheering forces, and introduce chaotropic salts that can carry over into the final sample, inhibiting downstream applications.
GenElute™-E kits employ size exclusion negative chromatography to separate large nucleic acid molecules from smaller protein, lipid, and ionic components in cell, tissue, blood, and other samples. Single-spin columns efficiently absorb and retain cellular debris and sample contaminants while allowing nucleic acids to pass through, reducing the number of steps and plastic materials required for purification. This novel method for high-quality purification is made possible by our innovative SmartLyse® Protease, which enables fast and efficient lysis of a wide range of sample types.
Isolate nucleic acid in a fraction of time compared to traditional silica bind-wash-elute procedures. Simply mix sample with lysis components and incubate according to kit protocol, pipette sample onto the spin column, and centrifuge directly into a collection tube. Cellular debris and contaminants remain bound in the column to be discarded, while purified DNA is ready to be used in downstream applications or stored.
- Isolation of DNA in under an hour with minimal sample handling
- Better purity, leading to improved performance in PCR and other applications
- Significant reduction in plastic and hazardous chemical waste
Traditional silica-based, bind-wash-elute purification kits require multiple wash steps to remove impurities from the spin columns. These steps increase the risk of cross contamination, subject the DNA to centrifugation sheering forces, and introduce chaotropic salts that can carry over into the final sample, inhibiting downstream applications.
GenElute™-E kits employ size exclusion negative chromatography to separate large nucleic acid molecules from smaller protein, lipid, and ionic components in cell, tissue, blood, and other samples. Single-spin columns efficiently absorb and retain cellular debris and sample contaminants while allowing nucleic acids to pass through, reducing the number of steps and plastic materials required for purification. This novel method for high-quality purification is made possible by our innovative SmartLyse® Protease, which enables fast and efficient lysis of a wide range of sample types.
Isolate nucleic acid in a fraction of time compared to traditional silica bind-wash-elute procedures. Simply mix sample with lysis components and incubate according to kit protocol, pipette sample onto the spin column, and centrifuge directly into a collection tube. Cellular debris and contaminants remain bound in the column to be discarded, while purified DNA is ready to be used in downstream applications or stored.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is aligned with "Waste prevention" and "Designing safer chemicals". Click here for more information.
Application
The GenElute™-E Cap Puncher is designed to work with the GenElute™-E line of DNA and RNA purification kits. When pressed onto the lid of the single-spin column, the cap puncher creates a small opening through which a sample can be pipetted directly onto the column. This reduces the amount of handling by eliminating the need for cap removal, and lessens the chance of cross contamination when handling multiple samples.For full instructions, please reference any of the GenElute™-E protocols.
Other Notes
We believe that green chemistry will contribute to a better tomorrow. With a growing portfolio of greener alternatives, there are now more choices to reduce the ecological impact of your research while still delivering quality and efficacy so your results are not compromised. GenElute™-E purification kits avoid the use of hazardous wash buffers typically employed in silica-based purification kits, significantly reducing chemical waste. The single-spin protocol also limits the number of collection steps, reducing plastic waste. GenElute™-E is designated a Greener Alternative Product based on the "Prevention" and "Designing Safer Chemicals" qualifications of The 12 Principles of Green Chemistry.GenElute™-E kits also adhere to the principles of SMASH Packaging, our global initiative to improve the sustainability through the reduction of packaging materials, the increased use of sustainable alternatives, and improved recyclability. Packaging is FCS-marked for sustainable forestry practices and produced from greater than 70% recycled content.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
SmartLyse is a registered trademark of Merck KGaA, Darmstadt, Germany
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当該品目 | |||
|---|---|---|---|
| description For use with Genelute™-E kits. 1 cap puncher. | description Reagents and consumables for various tissue sample types. 10, 50 or 250 purifications. | description Reagents and consumables for various plant sample types. 10, 50 or 250 purifications. | description Reagents and consumables for various plant sample types. 96-well plate format. |
| sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product |
| greener alternative product characteristics Waste Prevention | greener alternative product characteristics Waste Prevention | greener alternative product characteristics Waste Prevention | greener alternative product characteristics Waste Prevention |
| feature Intended use (For use with GenElute™-E Single Spin purification columns) | feature Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Typical/expected yield (Varies by sample. Please reference user guide for more information.), Intended use (For the purificiation of genomic DNA from human or animal tissue) | feature Intended use (For purification of genomic DNA from plant tissue), Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Typical/expected yield (Varies by sample. Please reference user guide for more information.) | feature Intended use (For purification of genomic DNA from plant tissue), Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Typical/expected yield (Varies by sample. Please reference user guide for more information.) |
| greener alternative category | greener alternative category | greener alternative category , Aligned | greener alternative category |
| storage temp. room temp | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| usage cap punching | usage - | usage - | usage - |
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Do we know how stable the purified DNA is through several freeze-thaw cycles?
1 回答-
役に立ちましたか？
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Does the technology introduce any bias into the sample?
1 回答-
GenElute™-E does not introduce biases that some “bind-wash-elute” technologies can add because the technology separates by size, rather than by what binds and what is released.
役に立ちましたか？
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What is the composition of the lysis buffer and clearing buffer after flowing through the resin?
1 回答-
The presence of EDTA, SDS, or excess salt can affect my PCR/ sequencing reaction. The lysis buffer information is proprietary, but we can say it is free of chaotropic salts. The resins are desalting resins so EDTA, SDS, and salts are depleted.
役に立ちましたか？
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Genelute-e single spin DNA and RNA purification kits use negative chromatographty to isolate nucleic acids. Can you explain how this approach simplifies workflows?
1 回答-
Instead of optimizing the bind, wash, and release steps in conventional silica-based spin purification preps, the technology focuses on a separation by performing a single step fractionation based on the size of the biomolecules, which results in depleted impurities.
役に立ちましたか？
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