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E5630

β-エストラジオール6-(O-カルボキシメチル)オキシム:BSA

mitogen-activated protein kinase (MAPK) stimulator, lyophilized powder

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あなたへ/カタログ番号在庫状況単価
5 mg
カートの在庫状況を確認する
¥49,500
10 mg

在庫from市川市大町368

¥64,200

この商品について

UNSPSC Code:
51111800
NACRES:
NA.77
MDL number:
Form:
lyophilized powder

¥49,500


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製品名

β-エストラジオール6-(O-カルボキシメチル)オキシム:BSA,

sterility

non-sterile

Quality Segment

form

lyophilized powder

extent of labeling

~30 mol steroid per mol BSA

solubility

phosphate buffer: 0.90-1.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow

shipped in

ambient

storage temp.

2-8°C

General description

β-Estradiol 6-(O-carboxy­methyl)oxime: BSA is an estradiol conjugate and is membrane-impermeable.[1] BSA is linked to the estradiol at position 6 to form the β-estradiol 6-(O-carboxymethyl) oxime: BSA conjugate.[1]

Application

β-Estradiol 6-(O-carboxy­methyl)oxime: BSA has been used:
  • to test its effect on the N-methyl-D-aspartate receptor (NMDAR) currents in female dorsal root ganglion (DRG) neurons[2]
  • to coat microplates for the detection of plasma 17β-Estradiol levels using indirect enzyme-linked immunosorbent assay (ELISA)[3]
  • to test its effect on MutL homolog 1 in colorectal cancer lines[4]

Biochem/physiol Actions

β-Estradiol 6-(O-carboxy­methyl)oxime: BSA mimics estrogen and favors amyloid precursor protein α (sAPPα) secretion in vitro studies with primary rat cortical neurons.[1] It also stimulates mitogen-activated protein kinase (MAPK) activity in human umbilical vein endothelial cells [5]

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当該品目
E6507K2126E1127
form

lyophilized powder

form

lyophilized powder

form

powder

form

powder

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

solubility

phosphate buffer: 0.90-1.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow

solubility

0.1 M phosphate buffer: 0.98-1.02 mg/mL, clear, yellow to orange (pH: 7.5)

solubility

ethanol: 19.60-20.40 mg/mL, clear, colorless to faintly yellow

solubility

0.1 M NaOH: 10 mg/mL, clear to slightly hazy, colorless to faintly yellow

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

sterility

non-sterile

sterility

-

sterility

-

sterility

non-sterile


pictograms

Health hazardExclamation mark

signalword

Warning

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 2

保管分類

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

E5630-5MG-PW: + E5630-25MG: + E5630-BULK: + E5630-100MG: + E5630-5MG: + E5630-1MG: + E5630-10MG-PW: + E5630-VAR: + E5630-10MG:

jan



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試験成績書(COA)

Lot/Batch Number

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質問

1–7/7 質問  
  1. Buongiorno, il datasheet incluso con il prodotto E56-30 non è chiaro. Potrei sapere il volume finale in cui sciogliere 5mg di E2/BSA e la relativa concentrazione?

    1 回答
    1. β-Estradiol 6-((O-carboxy-methyl)oxime: BSA, Product Number E5630, is soluble in PBS to 0.90-1.10 mg/mL, and this will yield a faintly hazy to hazy, colorless to faintly yellow solution. Accordingly, E5630-5MG is soluble in 4.5-5.6 mL of PBS. The actual batch-specific solubility is listed on the corresponding CoA. Please download the CoA from the “Documents” section of the PDP at the following link:
      https://www.sigmaaldrich.com/product/sigma/e5630#product-documentation

      役に立ちましたか?

  2. What is the solution stability of Product E5630, β-Estradiol 6-(O-carboxy­methyl)oxime: BSA?

    1 回答
    1. We have not performed solution stability studies, and have found no information on solution stability in the literature. We would therefore recommend using freshly prepared solutions.

      役に立ちましたか?

  3. How is Product E5630, β-Estradiol 6-(O-carboxy­methyl)oxime: BSA, prepared?

    1 回答
    1. Our method of preparation is considered proprietary information  However, a method of preparation (not necessarily Sigma-Aldrich’s) has been described in J. Biol. Chem., 228, 713-727 (1957).

      役に立ちましたか?

  4. What is the solubility of Product E5630, β-Estradiol 6-(O-carboxy­methyl)oxime: BSA?

    1 回答
    1. We have solubilized the product in phosphate buffered saline at 1 mg/mL.  Solutions may be somewhat hazy.

      役に立ちましたか?

  5. What is the Department of Transportation shipping information for this product?

    1 回答
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      役に立ちましたか?

  6. What is the shelf life of Product E5630, β-Estradiol 6-(O-carboxy­methyl)oxime: BSA?

    1 回答
    1. There is no assigned shelf life date for the product.  The guarantee of quality is one year from the time customer receives the product for unopened bottles stored at 2-8°C.

      役に立ちましたか?

  7. What is the molecular weight of Product E5630, β-Estradiol 6-(O-carboxy­methyl)oxime: BSA?

    1 回答
    1. The Certificate of Analysis has lot specific information for the number of moles of steroid per mole of bovine serum albumin.  From this data and the molecular weights of the components, an approximate molecular weight for the compound can be calculated.  For example, if the moles of beta estradiol to BSA is 32, then the approximate molecular weight for the compound is 76.4 kDa (BSA has a molecular weight of approximately 66.4 kDa and 32 moles of estradiol is approximately 10 kDa more).

      役に立ちましたか?

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