由来生物
rabbit
結合体
unconjugated
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
フォーム
buffered aqueous solution
分子量
antigen ~180 kDa
交差性
rat, mouse, human
濃度
~1.5 mg/mL
テクニック
indirect immunofluorescence: 5-10 μg/mL using NIH-3T3 cells
western blot: 2-4 μg/mL using HeLa cell lysate and PC12 cell lysate
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... DOCK1(1793)
詳細
Dedicator of cytokinesis protein 1 (DOCK1), also termed as DOCK180, is a member of a family of guanine nucleotide exchange factors (GEF). The DOCK1 gene is mapped to human chromosome 10q26.2.
特異性
Anti-DOCK1 (C-terminal region) specifically recognizes human, rat, and mouse DOCK1.
アプリケーション
Anti-DOCK1 (C-terminal region) antibody produced in rabbit has been used in immunoblotting[1] and immunofluorescence.
生物化学的／生理学的作用
Dedicator of cytokinesis protein 1 (DOCK1) acts as an activator of Rac and is involved in cytoskeletal reorganization and cell migration. By activating the β1 and αvβ5 integrins, DOCK1 regulates cell motility, filopodia formation, and phagocytosis. It is involved in the integrin signaling through the adapter molecule crk (Crk)- (p130) Crk-associated substrate p130 Cas complex. Its localization at focal adhesions induces the phosphorylation of p130Cas. DOCK1 forms a complex with engulfment and cell motility protein 1 (ELMO1) that functions as a bipartite guanine nucleotide exchange factor (GEF) for Rac and inhibits ubiquitylation of DOCK1. In mammalian cells, the CrkII-ELMO1-DOCK1 complex has been shown to promote phagocytosis. DOCK1 has been suggested to play a central role in netrin signaling during neuronal development by mediating the attractive responses by neurons to netrin-1. It mediates morphological changes such as integrin-mediated cell spreading and nerve growth factor (NGF)-induced neurite outgrowth. DOCK1 has been found to undergo ubiquitination and proteasome-dependent degradation, a process enhanced in response to epidermal growth factor (EGF), Crk, and adhesion-dependent signals.
物理的形状
0.01 M PBS溶液(pH 7.4, 15 mMアジ化ナトリウム含有)。
保管および安定性
Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
免責事項
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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最新バージョンのいずれかを選択してください：
Etsuko Kiyokawa et al.
Cell adhesion & migration, 3(3), 281-284 (2009-05-22)
DOCK180 is an atypical guanine nucleotide exchange factor of Rac1 identified originally as one of the two major proteins bound to the SH3 domain of the Crk adaptor protein. DOCK180 induces tyrosine phosphorylation of p130(Cas), and recruits the Crk-p130(Cas) complex
M L Albert et al.
Nature cell biology, 2(12), 899-905 (2001-01-09)
Integrin receptors are important for the phagocytosis of apoptotic cells. However, little is known about their function in mediating internalization, as previous studies used blocking antibodies for the inhibition of binding. Here we show that the alphavbeta5 receptor mediates both
Xiaoling Li et al.
Nature neuroscience, 11(1), 28-35 (2007-12-11)
Netrins are prototypical axon guidance cues whose attractive signaling requires the small GTPase Rac1. It remains unclear how Rac1 is regulated in the netrin pathway. DOCK180 is a member of a new family of guanine nucleotide exchange factors for Rho
Rapid aneuploidy diagnosis of partial trisomy 7q (7q34? qter) and partial monosomy 10q (10q26. 12? qter) by array comparative genomic hybridization using uncultured amniocytes
Chen CP, et al.
Taiwanese Journal of Obstetrics & Gynecology, 51(1), 93-99 (2012)
Yoshinori Makino et al.
Journal of cell science, 119(Pt 5), 923-932 (2006-02-24)
Dock180, a member of the CDM family of proteins, plays roles in biological processes such as phagocytosis and motility through its association with the signalling adaptor protein Crk. Recently, the complex formation between Dock180 and Elmo1 was reported to function
アクティブなフィルタ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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