CRISPR20V

Lenti CRISPR Universal Non-Target Control#3 Transduction Particles (LV04 vector)

• UNSPSCコード: 41106609
• NACRES: NA.51

特性

• 形状: liquid
• 品質水準: 200
• 包装: vial of 200 μL
• 濃度: (1x10⁶ TU/mL (via p24 titering assay))
• アプリケーション: CRISPR
• 輸送温度: dry ice
• 保管温度: −70°C

詳細

詳細

The Lenti CRISPR Non-Target Control#3 Transduction Particles (LV04 vector) include a gRNA sequence that does not target known human, mouse and rat genes. The non-target control#3 transduction particles are useful as a negative control in experiments using CRISPR lentiviral clones and for cell types that are not amenable to transfection. The Lenti CRISPR Non-Target Control#3 uses a dual-component system consisting of U6-driven guide RNA (non-target#3) and hPGK-driven Puromycin resistance cassette and Blue Fluorescence Protein for fluorophorescence. This control requires Cas9 be delivered by a separate vector.

Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging and envelope plasmids.

アプリケーション

Functional Genomics/Screening/Target Validation

その他情報

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively.

法的情報

CRISPR Use License Agreement

Lentiviral, WPRE, and Evrogen Licenses

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable