LB185

98062318, human blood, Lymphoblastoid

• UNSPSCコード: 41106514

特性

• 製品名: LB185, 98062318
• 由来生物: human blood
• 成長モード: Suspension
• 核型: Not specified
• 形態: Lymphoblastoid
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human lymphoblastoid, normal,EBV-transformed

細胞株の説明

The lymphocyte cell line LB185 has been established using mononuclear cells from peripheral blood of a 56 year old normal male. T cells from the same sample have been used as controls for cellular radiosensitivity studies. Cells have been subsequently transformed with EBV. Fibroblasts have been derived from the same individual and were designated 153BR (Sigma Catalogue number. 90011808). This has been confirmed by STR profiling at ECACC. Cells are lymphhoblastoid with floating aggregates present.

アプリケーション

Mutation studies

DNAプロファイル

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11
D16S539: 9
D5S818: 10,13
D7S820: 11
THO1: 8,9
TPOX: 8,10
vWA: 17,18

培地

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

継代と培養方法

Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO₂, 37°C. Cells form floating aggregates.

その他情報

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