SR-4987

94102704, mouse bone, Fibroblast-like

• UNSPSCコード: 41106514

特性

• product name: SR-4987, 94102704
• 由来生物: mouse bone
• 成長モード: Adherent
• 核型: Not specified
• 形態: Fibroblast-like
• 製品: Macrophage colony stimulating factor (M-MCF)
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse bone marrow stromal cells

細胞株の説明

A bone marrow-derived stromal cell line established by A Pessina in 1987 from a normal female 5 month old mouse. A bone marrow culture was treated with the supernatant from Y1 cells which actively produce viral C-particles (MuLV). This fibroblast-like cell line produces macrophage-colony stimulating factor (M-MCF) and exhibits a high sensitivity to fibroblast growth factor (FGF) in clonogenic assays. The cells produce sarcomas in syngenic mice and have a reported doubling time of 14.7 hours.

培地

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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