KYSE-70

94072012, human esophagus, Epithelial

• 別名: KY70 Cells, KYSE70 Cells
• UNSPSCコード: 41106514

特性

• product name: KYSE-70, 94072012
• 由来生物: human esophagus
• 成長モード: Adherent
• 核型: Hypotriploid with 8% polyploidy
• 形態: Epithelial
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

ヒト食道扁平上皮癌

細胞株の説明

Established from a poorly differentiated invasive oesophageal squamous cell carcinoma resected from middle intra-thoracic oesophagus of a 77-year-old Japanese man prior to treatment. This cell line has been mycoplasma eradicated at ECACC. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line. It is a known phenomenon that SSC cell lines can lose their Y chromosome.

培地

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

継代と培養方法

Split confluent cultures 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Doubling time ~35 hours.

その他情報

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