Derived from BALB/3T3 clone A31 (Sigma Catalogue number. 86110401) by infection with M-MSV. A M-MSV non-producer, but addition of MuLV permits rescue of the MSV genome. Cells produce tumours in suitable hosts; lack contact inhibition in vitro cultures; morphologically identical to virus releasing lines.
アプリケーション
Transformation and virus studies: M-MSV, MuLV
培地
DMEM + 2mM グルタミン + 10％ ウシ胎児血清（FBS）
継代と培養方法
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
その他情報
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試験成績書（COA）
Lot/Batch Number
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