A T lymphoblastoid line obtained from the peripheral blood of an 11 year old Caucasian male with acute lymphoblastoid leukaemia which has been passaged eight times through new-born Syrian Hamsters. These may be grown to a high density in a spinner type suspension culture.
Maintain cultures between 5-8x100,000 cells/ml; 5% CO2; 37°C. Maximum cell density 2-3x1,000,000 cells/ml.
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