3T3 Swiss Albino

85022108, mouse embryo, Fibroblast

• UNSPSCコード: 41106514

特性

• product name: 3T3 Swiss Albino, 85022108
• 由来生物: mouse embryo
• 成長モード: Adherent
• 核型: 2n = 40; hypertriploid
• 形態: Fibroblast
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

詳細

Product has lost contact inhibition

細胞株の由来

Mouse Swiss Albino embryo fibroblast

細胞株の説明

Evolved under conditions favouring retention of the property of contact inhibition of cell proliferation; they exhibit much more contact inhibition than diploid fibroblasts freshly plated in culture. Readily transformed by polyoma and SV40 viruses resulting in reduced sensitivity to contact inhibition. Cells have now lost their contact inhibition.

アプリケーション

Contact inhibition and virus studies: polyoma and SV40 viruses

培地

DMEM + 2mM グルタミン + 10％ ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 5x1,000 to 1x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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