B95a

1092505, cotton-top tamarin monkey blood, Not specified

• UNSPSCコード: 41106514

特性

• product name: B95a, from cotton-top tamarin monkey blood, 01092505, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• 由来生物: cotton-top tamarin monkey blood
• 成長モード: Semi-adherent
• 核型: Not specified
• 形態: Not specified
• 受容体: Not specified

詳細

細胞株の由来

B-lymphoblastoid, EBV-transformed

細胞株の説明

B95a is a derivative of the cell line B95-8 and was adapted to growth in adherence to the substrate surface. The parent line B95-8 was derived from a cotton-top tamarin (Saguinas oedipus). The B-lymphoblastoid cells are transformed with Epstein-Barr virus (EBV). B95a has been reported to be 10,000-fold more sensitive to measles virus present in clinical specimens than Vero cells and are useful host cells for the isolation of measles virus.

培地

DMEM (D6546) + 2 mM L-Glutamine (G7513) + 5% FBS / FCS (F2442) (for growth medium) or 2% FBS / FCS (F2442) (for maintenance medium).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 every 3-4 days using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C; cells grow initially as a monolayer, but will pile up and detach in maintenance medium.

その他情報

HPA Culture Collections由来でSIGMAによって供給される培養物は研究の目的のみに使用できます。商業目的での細胞株の使用に関する質問は、その細胞株のデポジターに照会されます。一部の細胞株には、細胞株の供給の前に受給予定者によって物質移動契約書への記入を完了させるという要求など、さらなる特別な公開条件があります。詳しくはTerms & Conditions of Supplyをご覧ください。