C7604

抗γ-チューブリン-Cy3^™ ウサギ宿主抗体

affinity isolated antibody, buffered aqueous solution

• 別名: 抗γ-チューブリン
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: CY3 conjugate
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen 48 kDa
• 化学種の反応性: human, chicken
• テクニック: direct immunofluorescence: 1:200 using methanol/acetone-fixed chicken fibroblats cells or HeLa cells (human).
• UniProtアクセッション番号: P23258
• 輸送温度: wet ice
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... TUBG1(7283) , TUBG2(27175)

詳細

詳細

γ-Tubulin (48 kDa) is a widely expressed and highly conserved protein within the microtubule organizing centers (MTOCs) or centrosome in eukaryotic cells.

Tubulin, γ 1 is a protein encoded by the TUBG1 gene in humans. This gene encodes a member of the tubulin superfamily. γ-tubulin interacts with Arp2/3, a complex that nucleates actin filaments and with the Arp2/3 activator WASH. It is found to have relationship with actin filament dynamics.

免疫原

ヒトγ-チュ-ブリンのN末端領域アミノ酸38-53に相当する合成ペプチド(C末端リジン付加)のKLH結合体。この配列はγ-チュ-ブリンに特異的であり、α-, β-, δ-, ε-チュ-ブリンなど他のチュ-ブリンファミリ-分子には認められません。この配列はマウスおよびラットγ-チュ-ブリンと同一であり、種属間で高度に保存されています(Drosophila, Aspergillus, 酵母γ-チュ-ブリンなど)。

アプリケーション

Anti-γ- Tubulin-Cy3 antibody produced in rabbit has been used in direct immunofluorescence (IF)and immunofluorescent staining.

Anti-γ-Tubulin-Cy3^™ antibody produced in rabbit is suitable for direct immunofluorescence at a dilution of 1:200 using methanol/acetone-fixed chicken fibroblats cells or HeLa cells (human).

生物化学的／生理学的作用

γ-Tubulin binds the microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome.

γ-Tubulins in the γ-TuRC might promote microtubule nucleation by providing a template that enhances the intrinsically weak lateral interaction between αβ-tubulin heterodimers when associated laterally. It has a unique functional property of a monomeric tubulin. γ-tubulin is also found to be expressed in lung cancer. Cep70 as an important centrosomal protein that interacts with γ-tubulin and plays a critical role in the regulation of mitotic spindle assembly.

物理的形状

0.01M PBS溶液 (pH 7.4, 1%BSA, 15mMアジ化ナトリウム含有)。

法的情報

Cy製品はAmersham Biosciences Limitedとのライセンス契約に基づいて販売しています。

Cy3 is a trademark of Cytiva

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable