C6423

α-Chymotrysin Protease

Cleaves at aromatic residues, suitable for mass spectrometry, from bovine pancreas

• CAS番号: 9004-07-3
• UNSPSC Code: 12352204
• eCl@ss: 42010112
• NACRES: NA.26
• EC Number: 232-671-2
• MDL number: MFCD00130481
• EC Number: 3.4.21.1 (BRENDA, IUBMB)

特性

• 製品名: α-キモトリプシン ウシ膵臓由来, suitable for protein sequencing, salt-free, lyophilized powder
• grade: Proteomics Grade
• Quality Segment: 200
• form: salt-free, lyophilized powder
• mol wt: 25 kDa
• suitability: suitable for protein sequencing
• UniProt accession no.: P00767
• storage temp.: 2-8°C
• Gene Information: cow ... CTRB1(618826)

詳細

General description

α-Chymotrypsin from bovine pancreas (bovine pancreatic α-chymotrypsin, CHT) is an enzyme protein.[1] The influence of varying concentrations of organic solvents like ethanol, 1,4-dioxane and acetonitrile on CHT has been reported.[2]

Chymotrypsin (Chy) is a serine protease. It corresponds to a molecular weight of 25.7 kDa and is widely used in pharmaceutical industry. It is synthesized in pancreas from chymotrypsinogen and require calcium for this conversion.

Application

α-Chymotrypsin from bovine pancreas is used for the following applications:

• Protein Identification by mass spectrometry (MS)[3]
• The isolation and characterization of myosin heavy chains[4]
• Toxin preparation[5]
• The incubation of infected and uninfected cells for analysis of cellular proteins by SDS-PAGE[6]

α-Chymotrypsin from bovine pancreas (BPC) may be used as a catalyst in the preparation of tetrahydroquinoline derivatives by Povarov reaction.

Biochem/physiol Actions

結合のカルボキシル基側にに芳香族側鎖または大きな疎水性側鎖(チロシン, トリプトファン, フェニルアラニン, メチオニン)を持つペプチド結合を加水分解するセリンプロテア-ゼです。

α-Chymotrypsin from bovine pancreas is stabilized by Ca²⁺ and catalyzes the hydrolysis of peptide bonds in particular the amino acids tyrosine, phenylalanine, tryptophan, and leucine at their C-terminal side. α-Chymotrypsin is inhibited by Cu²⁺ and Hg.

Other Notes

キモトリプリンに関する詳細な情報については、www.sigma-aldrich.com/enzymeexplorerをご覧ください。

1 unitは、pH 7.8、25°Cで1分間に1.0 μmolのBTEEを加水分解する酵素量です。

安全性情報

• pictograms: GHS08,GHS07,GHS09
• signalword: Danger
• hcodes: H302,H315,H319,H334,H335,H400
• pcodes: P261 - P264 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• 保管分類: 11 - Combustible Solids
• wgk: WGK 1
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves