C-29910
Freezing Medium Cryo-SFM
Serum-free medium for cryopreservation, 30 ml
別名:
Cryopreservation medium
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この商品について
NACRES:
NA.71
UNSPSC Code:
12352207
製品C-29910は現在お客様の国では販売されていません。 製品に関するお問い合わせはこちら（テクニカルサービス）
form
aqueous solution
packaging
pkg of 30 ml
technique(s)
cell culture | mammalian: suitable
shipped in
wet ice
storage temp.
2-8°C
General description
Cryo-SFM
Application
Cryo-SFM is a protein-free, defined and animal component-free cryopreservation medium. The optimized formulation is based on methylcellulose, DMSO and other cryoprotectants for the cryopreservation of human and animal primary cells. Cryo-SFM provides excellent results with all types of cells including primary human cells, stem cells and cell lines. Cells frozen in Cryo-SFM exhibit superior viability, attachment and subsequent growth performance after thawing. The medium is the first choice for all types of primary cells and cell lines.
Preparation Note
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Detach adherent cells using the PromoCell DetachKit (C-41200, C-41210, C-41220). Resuspend the cells in cold or room temperature Cryo-SFM at a concentration of 0.1 to 10 million cells/ml. Transfer the cell suspension into cryo-vials, freeze them gradually (-1°C/minute) to -70°C or lower overnight and subsequently store the vials in liquid nitrogen. Note: Each freeze/thaw cycle leads to a loss in proliferation potential and cell number, particularly when working with fragile primary cells. If freezing the cells, please do so at as low a passage number as possible.
Store Cryo-SFM at 4 to 8°C in the dark immediately after arrival. If stored properly, the product is stable until the expiry date stated on the label.
Analysis Note
All lots of PromoCell Cryo-SFM are subjected to comprehensive quality control tests. Each lot is routinely tested for its performance with primary human cells, for the absence of cytotoxicity, and for physical parameters such as osmolality and pH level. Approved in-house lots are used as a reference. In addition, all lots of Cryo-SFM have been tested for the absence of microbial contaminants (fungi, bacteria).
保管分類
12 - Non Combustible Liquids
一体何が起きているの？
WGK 1
引火点 (F)
Not applicable
引火点（°C）
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Substances Subject to be Indicated Names
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Substances Subject to be Notified Names
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C-29910:
jan