C267

モノクローナル抗μ-カルパイン(ドメインII)抗体 マウス宿主抗体

clone 2H2A7C2, ascites fluid

• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD01322346
• Conjugate: unconjugated
• Clone: 2H2A7C2, monoclonal
• Application: IF, WB
• Citations: 6

特性

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 2H2A7C2, monoclonal
• mol wt: antigen 80 kDa
• species reactivity: pig, rat, bovine, human
• technique(s): indirect immunofluorescence: 1:25, western blot: 1:1,000
• isotype: IgG1
• UniProt accession no.: P07384
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... CAPN1(823) ; rat ... Capn1(29153)

詳細

General description

Calpain-1 catalytic subunit is a protein encoded by the CAPN1 gene in humans. Calpains are calcium dependent proteases constituting a family of proteins. They share a homologous cysteine-protease domain and an E-F hand Ca²⁺-binding domain. The calpain system consists of two ubiquitous forms of calpain (m-calpain and μ-calpain), a tissue specific calpain (n-calpain) and a calpain inhibitory protein (calpastatin).

Immunogen

ウシ骨格筋由来μ-カルパイン。

Application

Monoclonal Anti-μ-Calpain (Domain II) antibody produced in mouse is suitable for indirect immunofluorescence at a dilution of 1:25 and western blotting at a dilution of 1:1000.

Biochem/physiol Actions

エピト-プマッピングの研究から、エピト-プはヒトμ-カルパインのアミノ酸245-265(ドメインII)の間にあることが示されています。μ-カルパインと特異的に反応する抗体です。m-カルパイン, n-カルパインやカルモジュリン、カルパスタチンとは反応しません。免疫沈降には適していません。イムノブロッティングにおいて、ヒト血小板, 赤血球、ウシ血小板, 心臓 ,骨格筋や、ラット筋芽細胞 ,腎臓 ,肝臓 ,脾臓サンプルとの反応性が観察されています。ブタLLC-PK1細胞の免疫蛍光染色では、細胞質が広く染色された様子が観察されています。

Calpain is involved in calmodulin-independent pathway for the activation of calcineurin. Endogenous calpain I forms active calcineurin in the human heart by proteolysis of calcineurin A, which may lead to pathogenesis of myocardial disease. μ-calpain, on overexpression, may have relationship with intractable epilepsy as well as the clinicopathological characteristics in such patients. Its activity may increase in skeletal muscle of gastric cancer patients.

Physical form

PBS溶液 (0.05%アジ化ナトリウム含有)。

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類: 10 - Combustible liquids
• 一体何が起きているの？: nwg
• 引火点 (F): Not applicable
• 引火点（°C）: Not applicable
• PPE（個人用保護具）: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)