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由来生物
mouse
結合体
biotin conjugate
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
PTR-8, monoclonal
形状
buffered aqueous solution
テクニック
direct ELISA: 1:40,000 using phosphothreonine-BSA at 10 μg/ml, and using ExtrAvidin-HRP at 2 μg/ml
dot blot: 1:120,000
アイソタイプ
IgG2b
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
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詳細
As determined by ELISA and dot blot, the antibody reacts specifically with phosphorylated threonine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated threonine, phosphoserine, phosphotyrosine, AMP or ATP. This antibody has been used in immunoblotting for the localization of some phosphothreonine-containing proteins. Certain proteins known to contain phosphorylated threonine may not be recognized by this antibody due to steric hindrance of the recognition site.
Monoclonal Anti-Phosphothreonine (mouse IgG2b isotype) is derived from the PTR-8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice.
免疫原
ホスホスレオニンのキ-ホ-ルリンペットヘモシアニン(KLH)結合体。
アプリケーション
Monoclonal Anti-Phosphothreonine−Biotin antibody produced in mouse has been used in phosphoprotein staining.
生物化学的／生理学的作用
Phosphorylation is a rare post-translational event in normal tissue. However, the abundance of phosphorylated cellular proteins increases several-fold following various activation processes. The residues phosphotyrosine, phosphoserine or phosphothreonine (p-Tyr/p-Ser/p-Thr) mediate such processes. Many signal transduction pathways, such as the epidermal growth factor (EGF) and insulin receptor systems, contain Tyr/Ser/Thr kinase which phosphorylates specific Tyr/Ser/Thr residues upon binding of ligands to their receptors. Monoclonal Anti-Phosphothreonine-Biotin may be used as an analytical tool for the identification and quantification of threonine-phosphorylated proteins
物理的形状
PBS溶液 (1%BSA, 15mMアジ化ナトリウム含有)。
保管および安定性
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers,is notrecommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
nwg
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
B7661-.5ML:
B7661-.2ML:
B7661-VAR:
B7661-BULK:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Jennifer L Miller et al.
Journal of proteome research, 8(10), 4789-4798 (2009-08-26)
Mitochondria, the powerhouse of eukaryotic cells, have their own translation machinery that is solely responsible for synthesis of 13 mitochondrially encoded protein subunits of oxidative phosphorylation complexes. Phosphorylation is a well-known post-translational modification in regulation of many processes in mammalian
Ya-Wei Zhao et al.
Scientific reports, 6, 34817-34817 (2016-10-05)
Phosphorylation is one of the most important protein post-translation modifications. With the rapid development of high-throughput mass spectrometry, phosphorylation site data is rapidly accumulating, which provides us an opportunity to systematically investigate and predict phosphorylation in proteins. The phosphorylation of
Fatima Ardito et al.
International journal of molecular medicine, 40(2), 271-280 (2017-06-29)
Protein phosphorylation is an impo-rtant cellular regulatory mechanism as many enzymes and receptors are activated/deactivated by phosphorylation and dephosphorylation events, by means of kinases and phosph-atases. In particular, the protein kinases are responsible for cellular transduction signaling and their hyperactivity
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