A9934
アミノペプチダーゼI Streptomyces griseus由来
lyophilized powder, ≥200 units/mg protein
別名:
ロイシンアミノペプチダーゼIV
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| あなたへ/カタログ番号 | 在庫状況 | 単価 |
|---|---|---|
0.1 mg | 在庫from市川市大町368 | ￥50,800 |
この商品について
CAS番号:
UNSPSC Code:
12352204
EC Number:
232-874-6
NACRES:
NA.54
MDL number:
Specific activity:
≥200 units/mg protein
￥50,800
在庫詳細
高品質はそのまま、さらにお手頃な価格になりました
form
lyophilized powder
Quality Segment
specific activity
≥200 units/mg protein
mol wt
21 kDa by gel filtration, 33 kDa by SDS-PAGE
composition
Protein, 40-60% Lowry
storage temp.
−20°C
General description
Aminopeptidase I from Streptomyces griseus is a thermostable enzyme with Glu131 and Tyr246 as key active site residues.[1]
Application
Biochem/physiol Actions
Aminopeptidase I from S. griseus has a fairly broad specificity, being able to remove the N-terminal residue of most proteins, except where the penultimate residue is an imino acid. It contains two Zn2+ binding sites. Aminopeptidase I from S. griseus is inhibited by 1,10-phenanthroline and is activated six-fold by Ca2+, which also stabilizes it against heat inactivation. This monomeric zinc metalloprotein has an isoelectric point (pI) of 5.4.
Aminopeptidase I may also be used as a reagent in the assay of endoprotease activities with a synthetic substrate in a two-stage assay. In the first stage, the endoprotease cleaves a peptide, such as Z-Y-X-Leu-p-nitroanilide, with the X, Y, and Z residues being chosen according to the specificity of the endoprotease.
Packaging
容量はタンパク質含有量に基づいています。
Physical form
酢酸カルシウムを含んでいます。
Preparation Note
Reconstitute in 20 mM tricine, pH 8.0, with 0.05% bovine serum albumin. Dilute the enzyme with the reconstitution buffer to 0.15-0.3 U/mL for a working concentration. Solutions should be prepared fresh prior to use.
Other Notes
1ユニットは、基質濃度3.0 mM、pH 8.0、25°C、1分間で、1.0 μmolのL-ロイシン-p-ニトロアニリドをL-ロイシンとp-ニトロアニリンに加水分解する酵素量です。
エンドペプチダ-ゼ混入物:0.01 U/mg未満:タンパク質(カゼインからの1分間放出量に相当するチロシンμmolとして。)
1 of 1
当該品目 | |||
|---|---|---|---|
| specific activity ≥200 units/mg protein | specific activity ≥3.5 units/mg solid | specific activity ≥5.0 units/mg protein | specific activity 0.5 units/mg protein |
| form lyophilized powder | form powder | form lyophilized powder | form solution |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt 21 kDa by gel filtration, 33 kDa by SDS-PAGE | mol wt - | mol wt 24.072 kDa by amino acid sequence, 28 kDa by SDS-PAGE | mol wt 37 kDa by SDS-PAGE |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| composition Protein, 40-60% Lowry | composition - | composition - | composition - |
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
保管分類
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)