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Merck

A9292

Sigma-Aldrich

抗-ウマIgG (全分子)−ペルオキシダーゼ ウサギ宿主抗体

IgG fraction of antiserum, buffered aqueous solution

別名:

Rabbit Anti-Horse IgG (whole molecule)−HRP

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About This Item

MDL番号:
UNSPSCコード:
12352203
NACRES:
NA.46

由来生物

rabbit

結合体

peroxidase conjugate

抗体製品の状態

IgG fraction of antiserum

抗体製品タイプ

secondary antibodies

クローン

polyclonal

形状

buffered aqueous solution

テクニック

direct ELISA: 1:15,000
dot blot: 1:100,000-1:200,000 (chemiluminescent)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150

輸送温度

dry ice

保管温度

−20°C

ターゲットの翻訳後修飾

unmodified

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詳細

Horse IgGs have seven subclasses ranging from IgG1 to IgG7. Equine IgG antibodies mainly regulate mucosal and systemic immunological responses and thereby, provide protection against disease-causing pathogens such as Streptococcus equi, and the horse flu virus. Horse IgG may also function to control the advancement of EHV-1 infection . Anti-horse IgG (whole molecule)−peroxidase antibody is specific for IgG in horses.

免疫原

Horse IgG

アプリケーション

Anti-horse IgG (whole molecule)−peroxidase antibody may be used in dot blot (1:8,000-1:10,000), immunohistochemistry (formalin-fixed, paraffin-embedded sections, at 1:150 dilution) and direct ELISA (1:15,000).
The level of S2-specific antibodies in test samples of horse serum was determined by ELISA using HRP-conjugated rabbit anti-horse IgG at a 1:35000 dilution with incubation for 45 minutes at room temperature.

生物化学的/生理学的作用

IgG, a monoclonal antibody can be cleaved at the hinge region by nonspecific proteases like papain and pepsin. This can result in univalent Fab (antigen-binding fragments) fragments or bivalent F(ab′)2 fragments.  These two enzymes have a broad substrate specificity resulting in heterogenous fragments.

物理的形状

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT

調製ノート

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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ピクトグラム

Health hazard

シグナルワード

Danger

危険有害性情報

危険有害性の分類

Resp. Sens. 1 - Skin Sens. 1

保管分類コード

12 - Non Combustible Liquids

WGK

WGK 2

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

A9292-BULK:
A9292-VAR:
A9292-2ML:4548173188126


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Ana Luísa Soares de Miranda et al.
Frontiers in veterinary science, 9, 852917-852917 (2022-06-18)
Loxosceles spp. (brown spiders) bites are responsible for the development of a syndrome consisting mainly of dermonecrotic lesions, and also systemic effects. Rabbits are one of the main experimental models used for better understanding the systemic and local effects of
Narongsak Chaiyabutr et al.
Veterinary world, 15(10), 2365-2375 (2022-11-26)
African horse sickness (AHS) is a non-contagious, high mortality, and insect-borne disease caused by a double-stranded RNA virus from the genus Orbivirus. The study aimed to develop inactivated vaccines serotype 1 inactivated AHS vaccine (IAV) and to compare the effect
Sha Jin et al.
Clinical and diagnostic laboratory immunology, 11(6), 1120-1129 (2004-11-13)
We recently reported a highly protective attenuated live virus vaccine for equine infectious anemia virus (EIAV) based on a proviral construct (EIAVUKDeltaS2) with a genetically engineered mutation in the viral S2 gene that eliminates expression of this accessory protein. While
Sabrina de Almeida Lima et al.
Frontiers in immunology, 9, 653-653 (2018-04-19)
Loxoscelism is the most important form of araneism in South America. The treatment of these accidents uses heterologous antivenoms obtained from immunization of production animals with crude loxoscelic venom. Due to the scarcity of this immunogen, new alternatives for its
Papain digestion of different mouse IgG subclasses as studied by electrospray mass spectrometry
Adamczyk M, et al.
Journal of Immunological Methods, 237(1-2), 95-104 (2000)

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