A4464

Enhanced Avian逆転写酵素 [eAMV^™RT]

For reverse transcription at higher temperatures & rare mRNAs

• UNSPSC Code: 12352204
• NACRES: NA.55
• MDL number: MFCD01634820
• Concentration: 20 units/μL

特性

• Quality Segment: 200
• form: crystalline powder
• usage: sufficient for 50 reactions
• feature: dNTPs included: no, hotstart: no
• concentration: 20 units/μL
• technique(s): RT-PCR: suitable, cDNA synthesis: suitable
• color: colorless
• input: purified RNA
• shipped in: wet ice
• storage temp.: −20°C

詳細

General description

eAMV^™ Reverse Transcriptase is an enhanced form of Avian Myeloblastosis Virus (AMV) RT that synthesizes a DNA strand complementary to RNA, DNA, or an RNA:DNA hybrid. This exceptionally robust AMV RT has greater thermostability than standard AMV or Moloney murine leukemia virus(M-MLV) reverse transcriptase. eAMV^™ RT is an ideal enzyme for producing high-quality full-length cDNA from total RNA or poly(A)⁺ RNA and is also efficient at transcribing long targets.

Application

Enhanced Avian (eAMV) Reverse Transcriptase is a highly purified, avian myeloblastosis virus reverse transcriptase (AMV-RT) that offers superior performance in comparison to standard AMV-RT or standard Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT).

This exceptionally robust AMV-RT has an enhanced ability to detect low abundance messages, transcribe through difficult secondary structure at elevated temperatures (up to 65 °C), and transcribe mRNA templates up to 14.1 kb.

Enhanced Avian Reverse Transcriptase (eAMV RT) is used to transcribe RNA into DNA, and facilitates efficient mRNA template driven sysnthesis of cDNAs. This is due to the abillity of this enhanced AMV-RT to transcribe large mRNA templates, to transcribe through difficult secondary structures, and to detect low abundance mRNAs by RT-PCR.

Enhanced Avian Reverse Transcriptase [eAMV^™ RT] has been used for reverse transcription of total RNA to synthesize cDNA during quantitative reverse transcription PCR (RT-qPCR) analysis.[1][2][3]

Biochem/physiol Actions

Reverse transcriptase catalyzes RNA template incorporation of dNTPs into complimantary DNA through phosphodiester bond formation.

Features and Benefits

• Greater sensitivity for low abundance mRNA
• Unsurpassed transcription through difficult secondary structures at elevated temperatures (up to 65°C)
• Efficient generation of full-length cDNA, up to 14.1 kb
• Produces first strand cDNA ready for PCR amplification

Packaging

Provided with a vial of 10× reaction buffer.

Other Notes

One unit incorporates one nanomole of TMP into TCA precipitable material in 10 min using polyadenylic acid as template and oligo(dT)_12-18 as a primer.

Legal Information

本製品の購入は研究のみを目的とし、サ-マルサイクラ-を使ってポリメラ-ゼ連鎖反応(PCR)処理を実施する場合に限ってその使用が認められます。自動化PCR処理に伴うサ-マルサイクラ-の利用は、Applied Biosystems社への支払いまたは正規に認められたサ-マルサイクラ-の購入による、ライセンス料の前払いによってカバ-されます。

eAMV is a trademark of Sigma-Aldrich Co. LLC

安全性情報

• 保管分類: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)