STR1

Enhanced Avian 一本鎖合成キット

Components for cDNA synthesis with enhanced AMV reverse transcriptase

• 別名: Reverse Transcriptase cDNA synthesis kit, eAMV-RT
• NACRES: NA.55
• UNSPSC Code: 12352200

特性

• Quality Segment: 200
• usage: sufficient for 50 reactions, kit sufficient for 50 reactions
• feature: dNTPs included, hotstart: no
• technique(s): RT-PCR: suitable, cDNA synthesis: suitable
• color: colorless
• input: purified RNA
• shipped in: dry ice
• storage temp.: −20°C

詳細

General description

Enhanced Avian First Strand Synthesis Kit utilizes a highly purified avian myeloblastosis virus reverse transcriptase (eAMV-RT) that offers superior performance in comparison to standard AMV-RT or standard Moloney murine leukemia virus reverse transcriptase (MMLV-RT). This exceptionally robust eAMV-RT has an enhanced ability to transcribe through difficult secondary structure at elevated temperatures (up to 65 °C) making it the ideal enzyme for producing high quality full-length cDNA from total RNA or poly(A)⁺ RNA.

Sigma′s Enhanced Avian RT First Strand Synthesis Kit also provides random nonamers (9-mers) and anchored oligo (dT)23 primers since gene-specific primers may not always be useful or possible. The anchored oligo has 23 thymidine residues and one G, C, or A residue (the anchor). The anchor ensures that the oligo (dT) primer binds at the very beginning of the message and that there is not a long region of the unusable sequence. With this kit, a dependable cDNA is generated that can be used for various downstream applications, including PCR.

Application

Enhanced Avian First Strand Synthesis Kit has been used for cDNA synthesis, which is intended for use in a real-time reverse transcription-polymerase chain reaction (RT-PCR).[1]

Other Notes

One unit incorporates one nanomole of TMP into TCA-precipitable material in 10 minutes using polyadenylic acid as template and oligo(dT)_12-18 as primer.

Legal Information

本製品の購入は研究のみを目的とし、サ-マルサイクラ-を使ってポリメラ-ゼ連鎖反応(PCR)処理を実施する場合に限ってその使用が認められます。PCRの自動化処理におけるサ-マルサイクラ-の利用は、Applied Biosystems社への支払いまたはライセンスを受けたサ-マルサイクラ-の購入による、ロイヤルティ-の前払いによってカバ-されます。

キットの構成要素のみ

• Enhanced Avian Reverse Transcriptase 1000 U

キットの構成要素は別途購入可能です。

• Deoxynucleotide mix 50 μL

  SDS
• O4387

  Anchored oligo (dT)₂₃ 100 μL

  SDS
• R7647

  Random nonamers 100 μL

  SDS
• O4387

  Ribonuclease inhibitor 50 μL

  SDS
• W1754

  PCR grade water 1.5 mL

  SDS

安全性情報

• 保管分類: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• signalword: Danger
• hcodes: EUH430
• pcodes: P201 - P202 - P273 - P391 - P405 - P501
• Hazard Classifications: ED ENV 1
• wgk: WGK 3